Hyperosmotic medium partially restores the growth defect and the impaired production of sterigmatocystin of an Aspergillus nidulans ΔpmtC mutant in a HogA-independent manner
| Autor: | Yumi Imanishi-Shimizu, Akira Yoshimi, Keietsu Abe, Thy Nhan Le, Thi Huynh Tram Le, Kiminori Shimizu |
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| Rok vydání: | 2021 |
| Předmět: |
Hyphal growth
Osmotic concentration Osmotic shock biology Chemistry Endoplasmic reticulum Sterigmatocystin Mutant Conidiation biology.organism_classification Microbiology Aspergillus nidulans Cell biology Culture Media Fungal Proteins Osmotic Pressure Gene Expression Regulation Fungal Mutation Genetics Osmotic pressure Molecular Biology |
| Zdroj: | FEMS microbiology letters. 368(18) |
| ISSN: | 1574-6968 |
| Popis: | The protein O-mannosyltransferase catalyzes O-mannosylation in the endoplasmic reticulum by transferring mannose to the seryl or threonyl residues of substrate proteins. We previously reported a deletion mutant of O-mannosyltransferase C (ΔpmtC) in Aspergillus nidulans with impaired vegetative growth and sterigmatocystin (ST) production. In this study, we investigated whether osmotic conditions contribute to the developmental processes and ST biosynthesis of the ΔpmtC deletion mutant. We found that hyphal growth and ST production partially improved in the presence of NaCl, KCl or sorbitol as osmotic stabilizers. Conidiation of the ΔpmtC deletion mutant was not restored under osmotic stress conditions when the hogA gene was deleted. The hogA gene encodes a protein required for the cellular response to osmotic pressure. However, the yield of ST and the vegetative growth of the ΔhogA ΔpmtC double deletant was restored by high osmolarity in a HogA-independent manner. |
| Databáze: | OpenAIRE |
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