Comparative genomics in Chlamydomonas and Plasmodium identifies an ancient nuclear envelope protein family essential for sexual reproduction in protists, fungi, plants, and vertebrates
| Autor: | David Goulding, Mathieu Brochet, Mandy Sanders, Gary Vanderlaan, Jimin Pei, Frank Schwach, Claudia Pfander, William J. Snell, Nick V. Grishin, Oliver Billker, Paul A. Lefebvre, Ellen Bushell, Thomas D. Otto, Jue Ning |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Plasmodium
Cell- och molekylärbiologi RNA sequencing fertilization Chlamydomonas KAR5/GEX1/Brambleberry nuclear envelope fusion 0302 clinical medicine Arabidopsis Genetics Plasmodium/genetics 0303 health sciences Genes Essential Chlamydomonas/genetics Reproduction Fungi/genetics/growth & development Nuclear Proteins Plants Transcriptome/genetics 3. Good health Reproduction/genetics Meiosis Fertilization/genetics Vertebrates Saccharomyces cerevisiae Proteins Protein family Isogamy Nuclear Envelope Biology 03 medical and health sciences parasitic diseases Animals Gene Plants/genetics Nuclear Proteins/classification/genetics/metabolism 030304 developmental biology Nuclear Envelope/metabolism Comparative genomics Arabidopsis Proteins Gene Expression Profiling Fungi Membrane Proteins Vertebrates/genetics biology.organism_classification Sexual reproduction Fertilization Saccharomyces cerevisiae Proteins/classification/metabolism Transcriptome Membrane Proteins/classification/metabolism 030217 neurology & neurosurgery Cell and Molecular Biology Resource/Methodology Arabidopsis Proteins/classification/metabolism Developmental Biology |
| Zdroj: | Genes and Development, Vol. 27, No 10 (2013) pp. 1198-1215 |
| ISSN: | 0890-9369 |
| Popis: | Fertilization is a crucial yet poorly characterized event in eukaryotes. Our previous discovery that the broadly conserved protein HAP2 (GCS1) functioned in gamete membrane fusion in the unicellular green alga Chlamydomonas and the malaria pathogen Plasmodium led us to exploit the rare biological phenomenon of isogamy in Chlamydomonas in a comparative transcriptomics strategy to uncover additional conserved sexual reproduction genes. All previously identified Chlamydomonas fertilization-essential genes fell into related clusters based on their expression patterns. Out of several conserved genes in a minus gamete cluster, we focused on Cre06.g280600, an ortholog of the fertilization-related Arabidopsis GEX1. Gene disruption, cell biological, and immunolocalization studies show that CrGEX1 functions in nuclear fusion in Chlamydomonas. Moreover, CrGEX1 and its Plasmodium ortholog, PBANKA_113980, are essential for production of viable meiotic progeny in both organisms and thus for mosquito transmission of malaria. Remarkably, we discovered that the genes are members of a large, previously unrecognized family whose first-characterized member, KAR5, is essential for nuclear fusion during yeast sexual reproduction. Our comparative transcriptomics approach provides a new resource for studying sexual development and demonstrates that exploiting the data can lead to the discovery of novel biology that is conserved across distant taxa. |
| Databáze: | OpenAIRE |
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