Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis

Autor: Aurélie Heuzé, Jean-Michel Hermel, Françoise Jamen, Gaëlle Recher, Philippe Herbomel, Thierry Savy, Nadine Peyriéras, Julia Jouralet, Emilie Mugniery, Sophie Desnoulez, Jean-Stéphane Joly, Franck Bourrat, Alessandro Brombin
Přispěvatelé: Neurobiologie et Développement (N&eD), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Institut de Neurobiologie Alfred Fessard (INAF), Centre National de la Recherche Scientifique (CNRS), INRA MSNC group Institut Fessard, CNRS, Institut National de la Recherche Agronomique (INRA), CHU Necker - Enfants Malades [AP-HP], Macrophages et Développement de l'Immunité, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut des Systèmes Complexes - Paris Ile-de-France (ISC-PIF), École normale supérieure - Cachan (ENS Cachan)-Université Panthéon-Sorbonne (UP1)-Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École polytechnique (X)-Institut Curie-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS) - Université Paris-Sud - Paris 11 (UP11), Hôpital Necker - Enfants malades, Assistance publique - Hôpitaux de Paris (AP-HP) - Université Paris Descartes - Paris 5 (UPD5), Institut Pasteur [Paris] - Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Cachan (ENS Cachan) - Université Panthéon-Sorbonne (UP1) - École normale supérieure - Paris (ENS Paris) - Université Paris-Sud - Paris 11 (UP11) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Polytechnique - X - INSTITUT CURIE - Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Cachan (ENS Cachan)-Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École polytechnique (X)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2013
Předmět:
Cell type
Embryo
Nonmammalian
Population
Ribosome biogenesis
Mitosis
Retina
Midbrain
03 medical and health sciences
neural stem cell
0302 clinical medicine
Neural Stem Cells
Mesencephalon
Morphogenesis
Animals
Gene Regulatory Networks
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Progenitor cell
education
Molecular Biology
Zebrafish
Cells
Cultured
030304 developmental biology
Cell Proliferation
Genetics
0303 health sciences
education.field_of_study
biology
Cell Cycle
Gene Expression Regulation
Developmental
Cell Differentiation
biology.organism_classification
Neural stem cell
Cell biology
nervous system
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
optic tectum
Zebrafish Information Network genome database
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Development (Cambridge, England)
Development (Cambridge, England), Company of Biologists, 2013, 140 (24), pp.4860-9. ⟨10.1242/dev.099010⟩
Development-Journal of embryology and experimental morphology
Development-Journal of embryology and experimental morphology, Company of Biologists, 2013, 140 (24), pp.4860-9. <10.1242/dev.099010>
Development (Cambridge, England), 2013, 140 (24), pp.4860-9. ⟨10.1242/dev.099010⟩
ISSN: 1477-9129
0950-1991
Popis: International audience; Investigating neural stem cell (NSC) behaviour in vivo, which is a major area of research, requires NSC models to be developed. We carried out a multilevel characterisation of the zebrafish embryo peripheral midbrain layer (PML) and identified a unique vertebrate progenitor population. Located dorsally in the transparent embryo midbrain, these large slow-amplifying progenitors (SAPs) are accessible for long-term in vivo imaging. They form a neuroepithelial layer adjacent to the optic tectum, which has transitory fast-amplifying progenitors (FAPs) at its margin. The presence of these SAPs and FAPs in separate domains provided the opportunity to data mine the ZFIN expression pattern database for SAP markers, which are co-expressed in the retina. Most of them are involved in nucleotide synthesis, or encode nucleolar and ribosomal proteins. A mutant for the cad gene, which is strongly expressed in the PML, reveals severe midbrain defects with massive apoptosis and sustained proliferation. We discuss how fish midbrain and retina progenitors might derive from ancient sister cell types and have specific features that are not shared with other SAPs.
Databáze: OpenAIRE
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