Consumption of barley ameliorates the diabetic steatohepatitis and reduces the high transforming growth factor β expression in mice grown in α-minimum essential medium in vitro as embryos
| Autor: | Kazuki Mochizuki, Atsushi Takahashi, Shiori Ishiyama, Nodoka Umihira, Teruhiko Wakayama, Mayu Kimura, Sachi Matsumoto, Satoshi Kishigami, Takao Nakagawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
QH301-705.5 Biophysics QD415-436 Biology Biochemistry Andrology 03 medical and health sciences 0302 clinical medicine Fibrosis Lipid droplet Barley medicine Biology (General) Non-alcoholic steatohepatitis Fatty liver nutritional and metabolic diseases Embryo Transforming growth factor beta medicine.disease In vitro Minimal essential medium 030104 developmental biology 030220 oncology & carcinogenesis embryonic structures biology.protein Steatohepatitis Transforming growth factor Non-alcoholic fatty liver disease |
| Zdroj: | Biochemistry and Biophysics Reports, Vol 27, Iss, Pp 101029-(2021) |
| ISSN: | 2405-5808 |
| Popis: | Non-alcoholic fatty liver disease (NAFLD), which includes the subtype non-alcoholic steatohepatitis (NASH), is a major complication of type 2 diabetic mellitus (T2DM), even among non-obese patients. However, the exact cause of NAFLD/NASH in non-obese patients with T2DM is unclear. We studied a non-obese mouse model of T2DM created through the malnourishment of embryos by culture in vitro for 48 h in α-minimum essential medium (MEM) at the two-cell stage. We compared the development of steatohepatitis in these MEM mice with control mice that were similarly cultured in standard potassium simplex-optimized medium (KSOM). We also studied the effects of 10 weeks of consumption of barley, which contains large amounts of the soluble fiber β-glucan, on the steatohepatitis of the adult MEM mice. The size of lipid droplets, the area of fibrosis, and the mRNA expression of the transforming growth factor beta (Tgfb) gene in the liver were higher in adult MEM mice fed a rice-based diet than in KSOM mice fed the same diet. However, barley consumption reduced the area of fibrosis and TGFB expression in MEM mice. In conclusion, adult mice that are cultured in MEM at the two-cell embryo stage develop steatohepatitis and T2DM, accompanied by higher hepatic TGFB expression, than KSOM controls. Furthermore, the consumption of barley during adulthood ameliorates the steatohepatitis and reduces the TGFB expression. |
| Databáze: | OpenAIRE |
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