Antiapoptotic serine protease inhibitors contribute to survival of allergenic TH2 cells
| Autor: | Jeff N. Temblay, Fedina Olexandra, Wei Cheng, Mohamed H. Shamji, Philip G. Ashton-Rickardt, Stephen R. Durham, Susan M. Byrne, Ellen Macfarlane, Natalia do Couto Francisco, Fabian Jacubczik, Amy Switzer |
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| Přispěvatelé: | Medical Research Council (MRC) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CD4(+) T-CELLS Male Allergy AIRWAY INFLAMMATION MEDIATED APOPTOSIS Small hairpin RNA memory Mice Immunology and Allergy ATOPIC-DERMATITIS RNA Small Interfering Cells Cultured Mice Knockout biology Innate lymphoid cell apoptosis Middle Aged 1107 Immunology Knockout mouse Pollen Female Inflammation Mediators Life Sciences & Biomedicine Adult Cell Survival Immunology TH2 MEMORY INNATE LYMPHOID-CELLS Serpin Poaceae Allergic inflammation 03 medical and health sciences Young Adult SERPIN Th2 Cells Antigens Neoplasm Hypersensitivity Animals Humans T(H)2 cells Serpins Serine protease Science & Technology CARCINOMA ANTIGEN-2 CATHEPSIN-G MEMORY CELLS Allergens Antigens Plant grass pollen allergy Molecular biology Mice Inbred C57BL Ovalbumin Disease Models Animal 030104 developmental biology Apoptosis biology.protein Immunologic Memory |
| Zdroj: | 581.e5 |
| Popis: | BACKGROUND: The mechanisms that regulate maintenance of persistent TH2 cells and potentiate allergic inflammation are not well understood. OBJECTIVE: The function of serine protease inhibitor 2A (Spi2A) was studied in mouse TH2 cells, and the serine protease inhibitor B3 (SERPINB3) and SERPINB4 genes were studied in TH2 cells from patients with grass pollen allergy. METHODS: Spi2A-deficient TH2 cells were studied in in vitro culture or in vivo after challenge of Spi2A knockout mice with ovalbumin in alum. Expression of SERPINB3 and SERPINB4 mRNA was measured in in vitro-cultured TH2 cells and in ex vivo CD27-CD4+ cells and innate lymphoid cell (ILC) 2 from patients with grass pollen allergy by using quantitative PCR. SERPINB3 and SERPINB4 mRNA levels were knocked down in cultured CD27-CD4+ cells with small hairpin RNA. RESULTS: There were lower levels of in vitro-polarized TH2 cells from Spi2A knockout mice (P |
| Databáze: | OpenAIRE |
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