Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage
| Autor: | Elke Raderschall, Thomas Haaf, Efim I. Golub |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
HMG-box
DNA repair DNA polymerase DNA damage Mitomycin DNA polymerase II DNA Single-Stranded Sensitivity and Specificity Cell Line chemistry.chemical_compound Animals Humans Cycloheximide Replication protein A Etoposide Cell Nucleus Mammals Xeroderma Pigmentosum Multidisciplinary DNA clamp biology Cell Cycle Nuclear Proteins Biological Sciences Fibroblasts Molecular biology Chromatin Recombinant Proteins Rats Kinetics Meiosis chemistry Gamma Rays Dactinomycin biology.protein DNA DNA Damage |
| Zdroj: | Proceedings of the National Academy of Sciences. 96:1921-1926 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | A sensitive and rapid in situ method was developed to visualize sites of single-stranded (ss) DNA in cultured cells and in experimental test animals. Anti-bromodeoxyuridine antibody recognizes the halogenated base analog incorporated into chromosomal DNA only when substituted DNA is in the single strand form. After treatment of cells with DNA-damaging agents or γ irradiation, ssDNA molecules form nuclear foci in a dose-dependent manner within 60 min. The mammalian recombination protein Rad51 and the replication protein A then accumulate at sites of ssDNA and form foci, suggesting that these are sites of recombinational DNA repair. |
| Databáze: | OpenAIRE |
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