Investigation of the genes associated with a male sterility mutant (msm) in Chinese cabbage (Brassica campestris ssp. pekinensis) using RNA-Seq
| Autor: | Xiang Li, Hui Feng, Runpeng Yao, Chong Tan, Shengnan Huang, Zhiyong Liu, Danyang Li, Shenling Peng, Li Hou, Chengyu Li |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Infertility Nuclear gene Sterility Stamen Genes Recessive Brassica Flowers Biology Genes Plant 01 natural sciences Genetic analysis 03 medical and health sciences Plant Growth Regulators Microspore Gene Expression Regulation Plant Exome Sequencing Genetics RNA-Seq Molecular Biology Gene Plant Proteins Gene Expression Profiling General Medicine 030104 developmental biology Pollen Pollen tube Transcriptome Pollen wall 010606 plant biology & botany |
| Zdroj: | Molecular Genetics and Genomics. 295:233-249 |
| ISSN: | 1617-4623 1617-4615 |
| Popis: | In Chinese cabbage, hybrid seed production is performed using male sterility lines, an important approach to heterosis utilization. In this study, a stably inherited male sterile mutant msm was obtained from the 'FT'-doubled haploid line of Chinese cabbage using isolated microspore culture combined with 60Co γ-ray mutagenesis. The genetic backgrounds of 'FT' and msm were highly consistent; however, compared with wild-type 'FT', msm exhibited completely degenerated stamens and no pollen phenotype. Other characters showed no significant differences. Cytological observations revealed that stamen abortion in msm begins during the tetrad period and that tapetum cells were abnormally expanded and highly vacuolated, leading to microspore abortion. Genetic analysis indicated that the msm mutant phenotype is controlled by a single recessive nuclear gene. Comparative transcriptome analysis of 'FT' and msm flower buds using RNA-Seq technology revealed 1653 differentially expressed genes, among which, a large number associated with male sterility were detected, including 64 pollen development- and pollen tube growth-related genes, 94 pollen wall development-related genes, 11 phytohormone-related genes, and 16 transcription factor-related genes. An overwhelming majority of these genes were down-regulated in msm compared with 'FT'. Furthermore, KEGG pathway analysis indicated that a variety of carbohydrate metabolic and lipid metabolic pathways were significantly enriched, which may be related to pollen abortion. The expression patterns of 24 male sterility-related genes were analyzed using qRT-PCR. In addition, 24,476 single-nucleotide polymorphisms and 413,073 insertion-deletion events were specifically detected in msm. These results will facilitate elucidation of the regulatory mechanisms underlying male sterility in Chinese cabbage. |
| Databáze: | OpenAIRE |
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