Bioanalytical method validation and sample analysis for nirmatrelvir in dried blood collected using the Tasso-M20 device

Autor:	Katty X, Wan, Daniel, Potts, Pilar, Gonzalez, Ian, Smith, Haihong, Shi, Olga, Kavetska
Rok vydání:	2022
Předmět:	Medical Laboratory Technology Lactams Tandem Mass Spectrometry Clinical Biochemistry Humans Reproducibility of Results Dried Blood Spot Testing General Medicine General Pharmacology Toxicology and Pharmaceutics Chromatography High Pressure Liquid Chromatography Liquid Analytical Chemistry
Zdroj:	Bioanalysis. 14:1305-1315
ISSN:	1757-6199 1757-6180
DOI:	10.4155/bio-2022-0167
Popis:	Aim: A sensitive and selective method for the determination of nirmatrelvir in dried human blood collected by Tasso-M20 was developed and validated from 20.0 to 20,000 ng/ml. Materials & methods: Nirmatrelvir and its stable-labeled internal standard were isolated from approximately 20 μl of blood dried on one volumetric absorptive pad inside the Tasso-M20 device by extraction with methanol, followed by dilution of the supernatant. The extracts were analyzed by high-performance liquid chromatography coupled with tandem mass spectrometric detection. Results & conclusion: The method was fully validated. Hematocrit levels do not impact assay accuracy. Stabilities to cover sample drying and storage at a variety of conditions were conducted. The validated method was used in multiple clinical studies with excellent performance.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c61a1b90bcaadb9e3655e728e629238 https://doi.org/10.4155/bio-2022-0167 Zobrazit plný text záznamu