Depression and Mania Induce Pro-inflammatory Activation of Macrophages Following Application of Serum from Individuals with Bipolar Disorder
| Autor: | Mariana Migliorini Parisi, Bruna Maria Ascoli, Florencia María Barbé-Tuana, Flávio Kapczinski, Pamela Ferrari, Fátima Theresinha Costa Rodrigues Guma, Adriane R. Rosa, Fábio Klamt, Rafael Colombo, Matheus Becker, Luiza Paul Géa, Gabriel Rodrigo Fries, Marcia Kauer-Sant Anna |
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| Rok vydání: | 2018 |
| Předmět: |
Chemokine
Bipolar disorder U-937 Proinflammatory cytokine 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Polarization medicine CXCL10 Pharmacology (medical) biology business.industry Brief Report Macrophages Monocyte Mononuclear phagocyte system medicine.disease 030227 psychiatry Psychiatry and Mental health medicine.anatomical_structure Immunology biology.protein Cytokines Tumor necrosis factor alpha Chemokines medicine.symptom business Mania 030217 neurology & neurosurgery |
| Zdroj: | Clinical Psychopharmacology and Neuroscience |
| ISSN: | 2093-4327 1738-1088 |
| DOI: | 10.9758/cpn.2018.16.1.103 |
| Popis: | Objective Evidence has suggested that immune imbalance is involved with bipolar disorder (BD); however, its precise mechanism is poorly understood. This study investigated whether biochemical changes in the serum from BD patients could modulate the phenotype of cultured macrophages. Methods Eighteen subjects with BD and five healthy individuals were included in this study. The human monocyte cell line U-937 was activated with phorbol 12-myristate 13-acetate (PMA) and polarization was induced with RPMI-1640 media supplemented with 10% serum from each patient for 24 hours. Gene expression of selected M1 and M2 markers was assessed by quantitative PCR. Results Macrophages exposed to serum of manic and depressive BD patients displayed an increase of interleukin-1β (6.40±3.47 and 9.04±5.84 vs. 0.23±0.11; p＜0.05) and tumor necrosis factor-α (2.23±0.91 and 2.03±0.45 vs. 0.62±0.24; p=0.002 and p=0.004, respectively) compared to euthymic group (there was no difference between euthymic and controls). In parallel, U-937 macrophages treated with serum of patients in acute episode displayed a down-regulation of CXCL9 (0.29±0.20 vs. 1.86±1.61; p=0.006) and CXCL10 expression (0.36±0.15 and 0.86±0.24 vs. 1.83±0.88; p＜0.000 and p=0.04) compared to the euthymia group. Conclusion Our results are consistent with previous studies showing that changes in peripheral blood markers could modulate M1/M2 polarization in BD. The evidence of macrophages as source of inflammatory cytokines might be helpful to unravel how the mononuclear phagocyte system is involved in the etiology of BD. |
| Databáze: | OpenAIRE |
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