Melatonin improves neuroplasticity by upregulating the growth-associated protein-43 (GAP-43) and NMDAR postsynaptic density-95 (PSD-95) proteins in cultured neurons exposed to glutamate excitotoxicity and in rats subjected to transient focal cerebral ischemia even during a long-term recovery period
| Autor: | Wei-Sheng Juan, Ai-Hua Lee, Che-Chao Chang, Tian Shung Wu, Ai-Chiang Lee, Tsung-Ying Chen, Sheng-Yang Huang, Yu-Chang Hung, Yu-Wen Lin, E-Jian Lee |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Dendritic spine Neurotoxins Excitotoxicity Glutamic Acid Biology medicine.disease_cause Neuroprotection Receptors N-Methyl-D-Aspartate Brain Ischemia Melatonin Rats Sprague-Dawley Endocrinology GAP-43 Protein Postsynaptic potential Internal medicine medicine Animals Cells Cultured Neurons Neuronal Plasticity Behavior Animal Glutamate receptor Intracellular Signaling Peptides and Proteins Brain Membrane Proteins Rats Up-Regulation Neuroprotective Agents NMDA receptor Postsynaptic density Neuroscience Disks Large Homolog 4 Protein medicine.drug |
| Zdroj: | Journal of pineal research. 56(2) |
| ISSN: | 1600-079X |
| Popis: | Recent evidence shows that the NMDAR postsynaptic density-95 (PSD-95), growth-associated protein-43 (GAP-43), and matrix metalloproteinase-9 (MMP-9) protein enhance neuroplasticity at the subacute stage of stroke. Here, we evaluated whether melatonin would modulate the PSD-95, GAP-43, and MMP-9 proteins in cultured neurons exposed to glutamate excitotoxicity and in rats subjected to experimental stroke. Adult male Sprague-Dawley rats were treated with melatonin (5 mg/kg) or vehicle at reperfusion onset after transient occlusion of the right middle cerebral artery (tMCAO) for 90 min. Animals were euthanized for Western immunoblot analyses for the PSD-95 and GAP-43 proteins and gelatin zymography for the MMP-9 activity at 7 days postinsult. Another set of animals was sacrificed for histologic and Golgi-Cox-impregnated sections at 28 days postinsult. In cultured neurons exposed to glutamate excitotoxicity, melatonin significantly upregulated the GAP-43 and PSD-95 expressions and improved dendritic aborizations (P |
| Databáze: | OpenAIRE |
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