Nitric oxide and prostaglandins are involved in the macula densa control of the renin system
| Autor: | Armin Kurtz, Karin Schricker, Marlies Hamann |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty Physiology Indomethacin Prostaglandin Arginine Nitric Oxide Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Furosemide Internal medicine Renin Renin–angiotensin system medicine Animals RNA Messenger Kidney Tubules Distal Kidney Juxtaglomerular apparatus Juxtaglomerular Apparatus Rats NG-Nitroarginine Methyl Ester Endocrinology medicine.anatomical_structure chemistry Eicosanoid Prostaglandins Macula densa Angiotensin I Nitric Oxide Synthase medicine.drug |
| Zdroj: | American Journal of Physiology-Renal Physiology. 269:F825-F830 |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.1995.269.6.f825 |
| Popis: | This study sought to examine the involvement of prostaglandins and of nitric oxide (NO) in the macula densa-dependent activation of the renin system in vivo. For this purpose, male Sprague-Dawley rats were chronically infused with furosemide (12 mg/day) for 6 days to inhibit macula densa salt transport. To inhibit the synthesis of prostaglandins and of NO, animals were injected with indomethacin (2 mg/kg twice daily) and with nitro-L-arginine methyl ester (L-NAME; 40 mg/kg twice daily) for the last 2 days of the experiment, respectively. Furosemide infusion increased plasma renin activity (PRA) from 8.8 +/- 1.4 to 41 +/- 5.2 ng angiotensin I (ANG I).h-1.ml-1 and renin mRNA levels from 112 +/- 8 of standard to 249 +/- 18% of standard. After treatment with indomethacin, the furosemide-induced increases in renin mRNA levels was attenuated to 190 +/- 11% of standard. After injections of L-NAME, both the furosemide-induced increases of renin mRNA levels and of PRA were reduced to 126 +/- 14% of standard and 22 +/- 5 ng ANG I.h-1.ml-1, respectively. These findings suggest that activation of renin gene expression by blockade of the macula densa function is dependent on intact NO and prostaglandin formation, whereas for stimulation of renin secretion mainly intact NO formation appears to be necessary. |
| Databáze: | OpenAIRE |
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