Comparative rates of hydrolysis of ochratoxins A and B in vitro
| Autor: | R.O. Sinnhuber, R.C. Doster |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Ochratoxin A
Carboxypeptidases In Vitro Techniques Toxicology Ochratoxins chemistry.chemical_compound Hydrolysis Coumarins Intestine Small medicine Animals Intestine Large Ochratoxin chemistry.chemical_classification Chromatography biology food and beverages Mycotoxins Carboxypeptidase Small intestine Rats medicine.anatomical_structure Enzyme Liver chemistry Biochemistry Spectrophotometry biology.protein Carboxypeptidase A Chromatography Thin Layer |
| Zdroj: | Food and Cosmetics Toxicology. 10:389-394 |
| ISSN: | 0015-6264 |
| DOI: | 10.1016/s0015-6264(72)80257-8 |
| Popis: | The rates of hydrolysis of ochratoxins A and B in vitro by the enzyme, bovine carboxypeptidase A, and by enzymes in tissue extracts of rat liver, small intestine and large intestine were studied. The Vmax values for the hydrolysis of ochratoxins A and B by carboxypeptidase are 5·15 × 10−7 and 4·35 × 10−5 moles/litre/min, respectively. Incubation of the extracts of rat tissue with an equimolar amount of ochratoxin A or B indicated that ochratoxin B was hydrolysed 6–7 times faster than ochratoxin A. It is suggested that this difference in hydrolysis rate may partly account for the difference in toxicity of ochratoxins A and B. |
| Databáze: | OpenAIRE |
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