Purification of Capsular Polysaccharide Produced by Streptococcus pneumoniae Serotype 19A
| Autor: | Bui Nguyet Minh, Eun Seong Seo, Hwa Ja Ryu, Doman Kim, Sang Il Yun, Sheng De Jin, Seung Jin Jung |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Serotype
chemistry.chemical_classification Streptococcus pneumoniae serotype biology Chemistry Polysaccharides Bacterial Serum Albumin Bovine General Medicine Chemical Fractionation medicine.disease_cause biology.organism_classification Streptococcaceae Polysaccharide Applied Microbiology and Biotechnology Microbiology Streptococcus pneumoniae Bacterial Proteins Nucleic Acids medicine Dialysis Bacteria Biotechnology |
| Zdroj: | Journal of Microbiology and Biotechnology. 21:734-738 |
| ISSN: | 1738-8872 1017-7825 |
| DOI: | 10.4014/jmb.1010.10043 |
| Popis: | Streptococcus pneumoniae is a major cause of invasive infection in young infants and older adults. There are currently 90 capsular serotypes identified and 23 serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19F, 19A, 20, 22F, 23F, and 33F) are responsible for about 90% of invasive disease. Among the more than 90 different S. pneumoniae serotypes, serotype 19A is globally very prevalent. A simplified purification procedure including adjustment of cell lysate pH to 4.5, fractionation with 50- 80% ethanol, and dialysis rendered capsular polysaccharide (CPS) in a yield of 31.32 +/- 3.11 mg from 1 l culture (75% recovery after lyses). The product contained only 69.6 microng of protein (99.78% purity) and 0.8 mg (sum of the precipitants from 50~60%, 60~70%, and 70~80%) of nucleic acid (97.45% purity). The purified CPS was conjugated with bovine serum albumin; the product size ranged from 100 to 180 kDa. |
| Databáze: | OpenAIRE |
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