Subacute oral administration of folic acid elicits anti-inflammatory response in a mouse model of allergic dermatitis
| Autor: | Naofumi Takahashi, Yuko Watanabe, Hitoshi Tajima, Ryoichi Ohtsuka, Yoshimasa Okazaki, Risako Tajiki-Nishino, Tomoki Fukuyama, Emi Makino, Aya Ohnuma-Koyama |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Keratinocytes
0301 basic medicine Allergy T-Lymphocytes Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Administration Oral Pharmacology Biochemistry 03 medical and health sciences Folic Acid 0302 clinical medicine Oral administration In vivo medicine Animals Humans 030212 general & internal medicine Megaloblastic anemia Molecular Biology Antipruritic Sensitization Cell Proliferation Mice Inbred BALB C Nutrition and Dietetics business.industry Anti-Inflammatory Agents Non-Steroidal Antipruritics medicine.disease Mice Inbred C57BL Disease Models Animal Toxicity Tests Subacute 030104 developmental biology Cytokine medicine.anatomical_structure Dermatitis Allergic Contact Female Lymph business medicine.drug |
| Zdroj: | The Journal of Nutritional Biochemistry. 67:14-19 |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/j.jnutbio.2019.01.009 |
| Popis: | Folic acid (FA) deficiency is associated with several health problems, including megaloblastic anemia and fetal neural tube defects. Therefore, supplementation with FA is strongly recommended by governments worldwide. Recent published reports indicate that FA functions in immune system maintenance. The main objective of this study is to examine possible anti-inflammatory and antipruritic effects of FA using a mouse model of allergic dermatitis. The mouse model was developed by repetitive sensitization to the Th2-type hapten toluene-2,4-diisocyanate (TDI). During the development of allergic dermatitis, FA was orally administered to the mice at doses of 8, 160, 1000 or 10,000 μg/day for 5 weeks. The ear swelling response and scratching behavior were monitored after the TDI challenge. Serum, ear tissue and auricular lymph node samples were isolated for further analysis 24 h after the TDI challenge. The ear swelling response was reduced in a dose-dependent manner by FA administration, and a significant change was observed at a concentration of 10,000-μg/day group. Comparable results were obtained through histological evaluation and cytokine level measurement in the ear tissue samples. Oral administration of FA exhibited the inhibitory effect on T-cell infiltration and T-cell-related cytokine production in auricular lymph nodes. Scratching behavior was not altered by FA administration. The in vivo evidence was corroborated by in vitro results, which showed that FA treatment significantly interfered with T-cell proliferation in a dose-dependent manner. Our findings imply that subacute oral administration of FA elicits an anti-inflammatory response, mainly through inhibition of T-cell proliferation. |
| Databáze: | OpenAIRE |
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