Special issue in honour of Prof. Reto J. Strasser - A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass)
| Autor: | H. Campos, F.V. Conde-Martínez, D. Padilla-Chacón, B. Jiménez-Francisco, C. Trejo, Carl J. Bernacchi, Govindjee Govindjee, A. Stirbet |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Chlorophyll a Sucrose Osmotic shock Physiology kinetic parameters of the o-j macromolecular substances Plant Science 01 natural sciences Performance index chemistry.chemical_compound lcsh:Botany and i-p phases osmotic stress performance index Photosystem biology food and beverages 04 agricultural and veterinary sciences biology.organism_classification Fluorescence lcsh:QK1-989 Horticulture On cells chemistry Bouteloua gracilis 040103 agronomy & agriculture j-i 0401 agriculture forestry and fisheries 010606 plant biology & botany |
| Zdroj: | Photosynthetica, Vol 58, Iss 2, Pp 262-274 (2020) |
| ISSN: | 1573-9058 0300-3604 |
| DOI: | 10.32615/ps.2019.148 |
| Popis: | Bouteloua gracilis (blue grama grass) is one of the most drought and grazing tolerant plants in the short-grass ecosystem. To obtain information on their photosystem activities, we measured the fast (< 1 s) chlorophyll a fluorescence transient (the OJIP curve) from their leaves, and isolated cells grown photoautotrophically in suspension in a culture medium, or with added sucrose. One of our goals was to study the effect of different sucrose concentrations (0, 0.15, 0.3, and 3%) on PSII activity in isolated cells. Our results on cells suspended in culture medium, using the JIP-test, showed a decrease in PSII activity at increasing sucrose concentrations, while the photoautotrophic cells showed an optimal PSII activity, close to that of the leaves. Further, our data on cells grown in 0, 0.15, and 0.3% sucrose, but with added CO2, and measured while the cells were deposited on filter paper, indicate that supplementary CO2 can increase the PSII activity in the presence of sucrose, although further research is necessary to understand these results. |
| Databáze: | OpenAIRE |
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