Cell kinetics of auxin transport and activity in Arabidopsis root growth and skewing
| Autor: | Jiří Friml, Qingguo Chen, Eilon Shani, Ohad Doron, Yun Hu, Yangjie Hu, Yunde Zhao, Or Megides, Ilan Tsarfaty, Ori Chekli, Moutasem Omary, Lukas Hoermayer, Zhaojun Ding |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cell kinetics Root growth Plant growth 1.1 Normal biological development and functioning Science Meristem Regulator Arabidopsis General Physics and Astronomy Plant Development 01 natural sciences Plant Roots Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Underpinning research Auxin heterocyclic compounds chemistry.chemical_classification Multidisciplinary biology Indoleacetic Acids Arabidopsis Proteins Intercellular transport fungi food and beverages General Chemistry biology.organism_classification Cell biology Kinetics 030104 developmental biology chemistry Oxygenases Root apical meristem 010606 plant biology & botany |
| Zdroj: | Nature communications, vol 12, iss 1 Nature Communications Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021) |
| Popis: | Auxin is a key regulator of plant growth and development. Local auxin biosynthesis and intercellular transport generates regional gradients in the root that are instructive for processes such as specification of developmental zones that maintain root growth and tropic responses. Here we present a toolbox to study auxin-mediated root development that features: (i) the ability to control auxin synthesis with high spatio-temporal resolution and (ii) single-cell nucleus tracking and morphokinetic analysis infrastructure. Integration of these two features enables cutting-edge analysis of root development at single-cell resolution based on morphokinetic parameters under normal growth conditions and during cell-type-specific induction of auxin biosynthesis. We show directional auxin flow in the root and refine the contributions of key players in this process. In addition, we determine the quantitative kinetics of Arabidopsis root meristem skewing, which depends on local auxin gradients but does not require PIN2 and AUX1 auxin transporter activities. Beyond the mechanistic insights into root development, the tools developed here will enable biologists to study kinetics and morphology of various critical processes at the single cell-level in whole organisms. Auxin gradients regulate plant root growth and development. Here the authors manipulate auxin synthesis in specific root cell types and use single-cell nucleus tracking and morphokinetics to map directional auxin flow in the root and quantify the kinetics of meristem skewing. |
| Databáze: | OpenAIRE |
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