Zooming in on the (peri)synaptic extracellular matrix
| Autor: | Alberto Diaspro, Francesca Cella Zanacchi, Alexander Dityatev, Svetlana Korotchenko |
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| Přispěvatelé: | Nägerl, U. Valentin, Triller, Antoine |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Neuromethods ISBN: 9781461491781 New York, NY : Springer New York, Neuromethods 84, 187-203 (2014). doi:10.1007/978-1-4614-9179-8_10 Nanoscale Imaging of Synapses / Nägerl, U. Valentin (Editor) ; New York, NY : Springer New York, 2014, Chapter 10 ; ISSN: 0893-2336=1940-6045 ; ISBN: 978-1-4614-9178-1=978-1-4614-9179-8 ; doi:10.1007/978-1-4614-9179-8 |
| DOI: | 10.1007/978-1-4614-9179-8_10 |
| Popis: | Neurons and glial cells secrete numerous molecules that accumulate in the extracellular space and form the neural extracellular matrix (ECM). There are diverse forms of the ECM. The most conspicuous aggregates of ECM molecules, containing chondroitin sulfate proteoglycans, hyaluronic acid, link proteins, and tenascin- R, are found around perisomatic GABAergic synapses on fast-spiking interneurons. However, also the perisynaptic extracellular space of glutamatergic synapses is enriched in ECM molecules such as hyaluronic acid and brevican. These ECM molecules and their receptors, the most studied of which are integrins, regulate the induction and maintenance of synaptic modifi cations. Recent data highlight the importance of synaptically secreted extracellular matrix molecule LGI1 in organization of synaptic machinery. Accumulated functional data point to the view that a synapse is a tetrapartite system including the pre- and postsynapse, associated astroglial terminals, and the ECM, in which there is an exchange of signals between all four components. This review is to give a short introduction to neural ECM; to summarize available electron microscopy data on expression of perisynaptic and synaptic ECM molecules and their receptors, as a reference for other super-resolution methods; and to present a protocol for STORM imaging of (peri) synaptic ECM using a commercially available super-resolution microscope (Nikon N-STORM). |
| Databáze: | OpenAIRE |
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