Effect of HPV on tumor expression levels of the most commonly used markers in HNSCC

Autor: Hana Binková, Jan Balvan, Jaromír Gumulec, Rom Kostrica, Martina Raudenská, Vojtech Adam, Michal Masarik, Michaela Fojtu, Hana Polanska, Zbynek Heger, René Kizek, Zuzana Horáková, Markéta Svobodová
Rok vydání: 2015
Předmět:
Male
0301 basic medicine
Pathology
MMP2
Cell
MMP9
0302 clinical medicine
Papillomaviridae
Aged
80 and over
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
HPV infection
virus diseases
General Medicine
Middle Aged
Prognosis
Survival Rate
Vascular endothelial growth factor A
medicine.anatomical_structure
Head and Neck Neoplasms
030220 oncology & carcinogenesis
Carcinoma
Squamous Cell
Female
Adult
medicine.medical_specialty
Real-Time Polymerase Chain Reaction
03 medical and health sciences
stomatognathic system
Biopsy
Biomarkers
Tumor
otorhinolaryngologic diseases
medicine
Humans
RNA
Messenger
neoplasms
Survival rate
Aged
Neoplasm Staging
business.industry
Gene Expression Profiling
Papillomavirus Infections
Head and neck cancer
medicine.disease
stomatognathic diseases
030104 developmental biology
Case-Control Studies
DNA
Viral
Cancer research
Neoplasm Grading
business
Follow-Up Studies
Zdroj: Tumor Biology. 37:7193-7201
ISSN: 1423-0380
1010-4283
DOI: 10.1007/s13277-015-4569-6
Popis: Approximately 90 % of head and neck cancers are squamous cell carcinomas (HNSCC), and the overall 5-year survival rate is not higher than 50 %. There is much evidence that human papillomavirus (HPV) infection may influence the expression of commonly studied HNSCC markers. Our study was focused on the possible HPV-specificity of molecular markers that could be key players in important steps of cancerogenesis (MKI67, EGF, EGFR, BCL-2, BAX, FOS, JUN, TP53, MT1A, MT2A, VEGFA, FLT1, MMP2, MMP9, and POU5F). qRT-PCR analysis of these selected genes was performed on 74 biopsy samples of tumors from patients with histologically verified HNSCC (22 HPV-, 52 HPV+). Kaplan-Meier analysis was done to determine the relevance of these selected markers for HNSCC prognosis. In conclusion, our study confirms the impact of HPV infection on commonly studied HNSCC markers MT2A, MMP9, FLT1, VEGFA, and POU5F that were more highly expressed in HPV-negative HNSCC patients and also shows the relevance of studied markers in HPV-positive and HPV-negative HNSCC patients.
Databáze: OpenAIRE
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