Establishment of an African green monkey model for COVID-19 and protection against reinfection
| Autor: | Stephanie L. Foster, Thomas W. Geisbert, John C. Heymann, Daniel J. Deer, Krystle N. Agans, Viktoriya Borisevich, Liana Medina, Corri B. Levine, Kevin Melody, Natalie S. Dobias, Joan B. Geisbert, Abhishek N. Prasad, Karla A. Fenton, Courtney Woolsey, Robert W. Cross |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
viruses T-Lymphocytes Immunology Gene Expression Antibodies Viral Article Natural killer cell Pathogenesis 03 medical and health sciences 0302 clinical medicine Chlorocebus aethiops medicine Coagulopathy Immunology and Allergy Animals Humans Epidemics medicine.diagnostic_test biology SARS-CoV-2 Gene Expression Profiling Respiratory disease Antibody titer COVID-19 medicine.disease 3. Good health Killer Cells Natural Disease Models Animal 030104 developmental biology Bronchoalveolar lavage medicine.anatomical_structure Reinfection biology.protein African Green Monkey Interferons Antibody 030215 immunology |
| Zdroj: | Nature immunology bioRxiv article-version (status) pre article-version (number) 1 Nature Immunology |
| ISSN: | 1529-2916 1529-2908 |
| Popis: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for an unprecedented global pandemic of COVID-19. Animal models are urgently needed to study the pathogenesis of COVID-19 and to screen vaccines and treatments. We show African green monkeys (AGMs) support robust SARS-CoV-2 replication and develop pronounced respiratory disease, which may more accurately reflect human COVID-19 cases than other nonhuman primate species. SARS-CoV-2 was detected in mucosal samples, including rectal swabs, as late as 15 days post-exposure. Marked inflammation and coagulopathy in blood and tissues were prominent features. Transcriptome analysis demonstrated stimulation of interferon and interleukin 6 pathways in bronchoalveolar lavage samples and repression of natural killer cell- and T cell-associated transcripts in peripheral blood. Despite a slight waning in antibody titers after primary challenge, enhanced antibody and cellular responses contributed to rapid clearance after re-challenge with an identical strain. These data support the utility of AGM for studying COVID-19 pathogenesis and testing medical countermeasures. One Sentence Summary: African Green monkeys infected with SARS-CoV-2 recapitulate pathological features of human COVID-19. |
| Databáze: | OpenAIRE |
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