| Autor: |
Regilme, Maria Angenica Fulo, Sato, Megumi, Tamura, Tsutomu, Arai, Reiko, Sato, Marcello Otake, Ikeda, Sumire, Doi, Masaya, Tanaka, Kohki, Gamboa, Maribet, Monaghan, Michael T., Watanabe, Kozo |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| DOI: |
10.1101/862904 |
| Popis: |
Ixodid tick species such asIxodes ovatusandHaemaphysalis flavaare essential vectors of tick-borne diseases in Japan. In this study, we investigated the population genetic structures and gene flow ofI. ovatusandH. flavaas affected by the tick host mobility. We hypothesized thatI. ovatusandH. flavamay have differences in their genetic structure due to the low mobility of small rodent hosts ofI. ovatusat the immature stage in contrast to the mediated dispersal of avian hosts for immatureH. flava.We collected 307 adultI. ovatusand 220 adultH. flavafrom 29 and 17 locations across Niigata Prefecture, Japan. We investigated the genetic structure at two mitochondrial loci (cox1, 16S rRNA gene). ForI. ovatus, pairwiseFSTand analysis of molecular variance (AMOVA) analyses ofcox1sequences indicated significant genetic variation among populations. Bothcox1and 16S rRNA markers showed non-significant genetic variation among locations forH. flava. The Bayesian tree and haplotype network ofcox1marker forI. ovatussamples in Niigata Prefecture found 3 genetic groups wherein most haplotypes in group 2 were distributed in low altitudinal areas. When we addedcox1sequences ofI. ovatusfrom China to the phylogenetic analysis, three genetic groups (China 1, China 2, and Niigata and Hokkaido, Japan) were formed in the tree suggesting the potential for cryptic species in the genetic group in Japan. Our results support our hypothesis and suggest that the host preference of ticks at the immature stage may influence the genetic structure and gene flow of the ticks. This information is vital in understanding the tick-host interactions in the field to better understand the tick-borne disease transmission and in designing an effective tick control program. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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