Characterization of photoreceptor degeneration in the rhodopsin P23H transgenic rat line 2 using optical coherence tomography

Autor: Sei-ichi Ishiguro, Natsuki Monai, Mitsuru Nakazawa, Reiko Tanabu, Kodai Yamauchi, Takayuki Gonome
Rok vydání: 2018
Předmět:
Photoreceptors
0301 basic medicine
Retinal degeneration
Pathology
Sensory Receptors
genetic structures
lcsh:Medicine
Social Sciences
Diagnostic Radiology
Rats
Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Psychology
lcsh:Science
Tomography
Neurons
Multidisciplinary
medicine.diagnostic_test
Chemistry
Radiology and Imaging
Retinal Degeneration
Organ Size
Photoreceptor outer segment
medicine.anatomical_structure
Disease Progression
Regression Analysis
Retinal Disorders
Sensory Perception
Rats
Transgenic
Cellular Types
Anatomy
Tomography
Optical Coherence
Retinitis Pigmentosa
Photoreceptor inner segment
Photoreceptor Cells
Vertebrate
Research Article
Signal Transduction
medicine.medical_specialty
Histology
Imaging Techniques
Ocular Anatomy
Outer plexiform layer
Research and Analysis Methods
Retina
03 medical and health sciences
Diagnostic Medicine
Ocular System
Electroretinography
Genetics
medicine
Animals
Point Mutation
Outer nuclear layer
lcsh:R
Retinitis
Biology and Life Sciences
Afferent Neurons
Retinal
Cell Biology
medicine.disease
eye diseases
Disease Models
Animal
Ophthalmology
030104 developmental biology
Cellular Neuroscience
Multivariate Analysis
Mutation
030221 ophthalmology & optometry
Eyes
lcsh:Q
sense organs
Head
Neuroscience
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 3, p e0193778 (2018)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0193778
Popis: Purpose To characterize the optical coherence tomography (OCT) appearances of photoreceptor degeneration in the rhodopsin P23H transgenic rat (line 2) in relation to the histological, ultrastructural, and electroretinography (ERG) findings. Materials and methods Homozygous rhodopsin P23H transgenic albino rats (line 2, very-slow degeneration model) were employed. Using OCT (Micron IV®; Phoenix Research Labs, Pleasanton, CA, USA), the natural course of photoreceptor degeneration was recorded from postnatal day (P) 15 to P 287. The OCT images were qualitatively observed by comparing them to histological and ultrastructural findings at P 62 and P 169. In addition, each retinal layer was quantitatively analyzed longitudinally during degeneration, compared it to that observed in wild type Sprague-Dawley (SD) rats. The relationships between the ERG (full-field combined rod-cone response, 3.0 cds/m2 stimulation) findings and OCT images were also analyzed. Results In the qualitative study, the two layers presumably corresponding to the photoreceptor inner segment ellipsoid zone (EZ) and interdigitation zone (IZ) were identified in the P23H rat until PN day 32. However, the photoreceptor inner and outer segment (IS/OS) layer became diffusely hyperreflective on OCT after P 46, and the EZ and IZ zones could no longer be identified on OCT. In contrast, in the SD rats, the EZ and IZ were clearly distinguished until at least P 247. The ultrastructural study showed partial disarrangements of the photoreceptor outer segment discs in the P23H rats at P 62, although a light-microscopic histological study detected almost no abnormality in the outer segment. In the quantitative study, the outer retinal layer including the outer plexiform layer (OPL) and the outer nuclear layer (ONL) became significantly thinner in the P23H rats than in the SD rats after P 71. The thickness of the IS/OS layer was maintained in the P23H rats until P 130, and it became statistically thinner than in the SD rats at P 237. The longitudinal attenuation in the amplitude of the a- and b-waves of ERG was significantly correlated with the thickness of the combined OPL and ONL but not with that of the IS/OS layer. Conclusion OCT showed the degenerated photoreceptor IS/OS layer in rhodopsin P23H transgenic rats (line 2) as a diffuse hyperreflective zone, even in the early stage, with the partially disarranged and destabilized OS discs recognizable by ultrastructural assessment but not by a histological study. The amplitude of the a- and b-waves mainly depends on the thickness of the OPL and ONL layer rather than the thickness of the photoreceptor IS/OS layer in P23H rats.
Databáze: OpenAIRE
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