The CWB2 Cell Wall-Anchoring Module Is Revealed by the Crystal Structures of the Clostridium difficile Cell Wall Proteins Cwp8 and Cwp6
| Autor: | Jure Borišek, Gregor Pretnar, Miha Renko, Aleksandra Usenik, Nataša Lindič, Andrej Perdih, Dušan Turk, Marko Mihelič, Uwe Müller |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Models
Molecular 0301 basic medicine 030106 microbiology Trimer Plasma protein binding Biology Crystallography X-Ray Bacterial cell structure Conserved sequence Cell wall 03 medical and health sciences Bacterial Proteins Cell Wall Structural Biology Hydrolase Binding site Cell adhesion Molecular Biology Conserved Sequence Binding Sites Clostridioides difficile Protein Structure Tertiary 030104 developmental biology Biochemistry Biophysics Protein Binding |
| Zdroj: | Structure. 25:514-521 |
| ISSN: | 0969-2126 |
| Popis: | Bacterial cell wall proteins play crucial roles in cell survival, growth, and environmental interactions. In Gram-positive bacteria, cell wall proteins include several types that are non-covalently attached via cell wall binding domains. Of the two conserved surface-layer (S-layer)-anchoring modules composed of three tandem SLH or CWB2 domains, the latter have so far eluded structural insight. The crystal structures of Cwp8 and Cwp6 reveal multi-domain proteins, each containing an embedded CWB2 module. It consists of a triangular trimer of Rossmann-fold CWB2 domains, a feature common to 29 cell wall proteins in Clostridium difficile 630. The structural basis of the intact module fold necessary for its binding to the cell wall is revealed. A comparison with previously reported atomic force microscopy data of S-layers suggests that C. difficile S-layers are complex oligomeric structures, likely composed of several different proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|