MicroRNA-mediated signal amplification coupled with GNP/dendrimers on a mass-sensitive biosensor and its applications in intracellular microRNA quantification
| Autor: | Hong-Yuan Chen, Yujie Wang, Yingshu Guo, Guangxu Yang, Jing-Juan Xu |
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| Rok vydání: | 2016 |
| Předmět: |
Dendrimers
Biomedical Engineering Biophysics Metal Nanoparticles Nanotechnology Biosensing Techniques 02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Dendrimer Electrochemistry Humans Chemistry Hybridization probe General Medicine 021001 nanoscience & nanotechnology Linker DNA 0104 chemical sciences MicroRNAs Colloidal gold Covalent bond MCF-7 Cells Quartz Crystal Microbalance Techniques Gold DNA Probes 0210 nano-technology Biosensor Linker DNA Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 85:897-902 |
| ISSN: | 0956-5663 |
| Popis: | Here, a mass-sensitive microRNA sensing surface is developed by utilizing a probe DNA/gold nanoparticles (GNP)/dendrimer composite coupling with an enzymatic amplification process. The probe DNA/GNP/dendrimer composite is prepared via the covalent coupling between the NH2 groups in PAMAM or DNA and the COOH group on GNP. Target microRNA binds to a stem-loop-structured DNA on maganatic NPs, forming a heteroduplex. By enzyme recycling amplification, a large number of linker sequences are produced on MNPs. Via the combination of probe DNA, the linker DNA on MNPs and a capture DNA on gold chip, the DNA/GNP/dendrimer probe could be assembled on the surface of gold chip inducing a sensitive frequency response. It presents an excellent performance in microRNA-203 quantification with a detection linear range of 1.0×10(-11)-1.0×10(-9)M. Both the success in discriminating expressing miroRNA-203 in MCF-7 cell and the well agreement with the commercial qRT-PCR detection method implied its potential application in early cancer diagnosis for future. |
| Databáze: | OpenAIRE |
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