Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals
| Autor: | Danica Agbaba, Slavko Marković, Breda Simonovska, Irena Vovk, Gordana Popović, Branislava Miseljic |
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| Předmět: |
Pharmacology
Chromatography Correlation coefficient 010405 organic chemistry Chemistry Elution 010401 analytical chemistry Analytical chemistry 01 natural sciences Dosage form 0104 chemical sciences Analytical Chemistry Volumetric flow rate Chromatography detector Impurity Phase (matter) medicine Environmental Chemistry Drug Contamination Agronomy and Crop Science Chromatography High Pressure Liquid Norfloxacin Food Science medicine.drug |
| Zdroj: | Scopus-Elsevier Journal of AOAC International Web of Science |
| Popis: | A gradient reversed-phase column high-performance liquid chromatographic method was developed for the detection and quantification of norfloxacin and its major impurities in norfloxacin-containing pharmaceuticals. Chromatographic separations were performed under the following experimental conditions: column, Zorbax SB RP-18 (5 m, 250 4.6 mm); injection volume, 20 L; mobile phase, 0.05 M NaH2PO4 (pH 2.5)acetonitrile (87 + 13) for 16 min and (58 + 42) for 9 min (stepwise gradient); and flow rate, 1.3 mL/min. All analyses were performed at 25C, and the eluate was monitored at 275 nm using a diode array detector. Linearity (correlation coefficient = 0.999), recovery (99.3101.8), relative standard deviation (0.20.7), and quantitation limit (0.120.47 g/mL) were evaluated and found to be satisfactory. The method is simple, rapid, and convenient for purity control of norfloxacin in both raw materials and dosage forms. |
| Databáze: | OpenAIRE |
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