Methyl-esterified 3-hydroxybutyrate oligomers protect bacteria from hydroxyl radicals
| Autor: | Ari Turpeinen, Jong H. Kim, Olga Podolich, Janne J. Koskimäki, Natalia Kozyrovska, Anna Maria Pirttilä, Mirva Pääkkönen, Emmi-Leena Ihantola, Marko Suokas, Bruce C. Campbell, Marena Kajula, Sampo Mattila, Elina Hankala, Juho Hokkanen, Heidi Hautajärvi, Johanna Pohjanen |
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| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
Radical Iron Hydroxybutyrates medicine.disease_cause Oligomer Antioxidants Gene Expression Regulation Enzymologic 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Methylobacterium extorquens medicine Molecular Biology Plant Diseases chemistry.chemical_classification Reactive oxygen species biology Molecular Structure Hydroxyl Radical Cell Biology Glutathione Gene Expression Regulation Bacterial Hydrogen Peroxide biology.organism_classification Pinus 030104 developmental biology Monomer chemistry Biochemistry Seedlings Hydroxyl radical Bacteria Oxidative stress |
| Zdroj: | Nature chemical biology. 12(5) |
| ISSN: | 1552-4469 |
| Popis: | Bacteria rely mainly on enzymes, glutathione and other low-molecular weight thiols to overcome oxidative stress. However, hydroxyl radicals are the most cytotoxic reactive oxygen species, and no known enzymatic system exists for their detoxification. We now show that methyl-esterified dimers and trimers of 3-hydroxybutyrate (ME-3HB), produced by bacteria capable of polyhydroxybutyrate biosynthesis, have 3-fold greater hydroxyl radical-scavenging activity than glutathione and 11-fold higher activity than vitamin C or the monomer 3-hydroxybutyric acid. We found that ME-3HB oligomers protect hypersensitive yeast deletion mutants lacking oxidative stress-response genes from hydroxyl radical stress. Our results show that phaC and phaZ, encoding polymerase and depolymerase, respectively, are activated and polyhydroxybutyrate reserves are degraded for production of ME-3HB oligomers in bacteria infecting plant cells and exposed to hydroxyl radical stress. We found that ME-3HB oligomer production is widespread, especially in bacteria adapted to stressful environments. We discuss how ME-3HB oligomers could provide opportunities for numerous applications in human health. |
| Databáze: | OpenAIRE |
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