Cationic lipopolymeric nanoplexes containing the CRISPR/Cas9 ribonucleoprotein for genome surgery

Autor:	Deepak Kumar Sahel, Mohd Salman, Mohd Azhar, Sangam Giri Goswami, Vivek Singh, Manu Dalela, Sujata Mohanty, Anupama Mittal, Sivaprakash Ramalingam, Deepak Chitkara
Rok vydání:	2022
Předmět:	HEK293 Cells Ribonucleoproteins Biomedical Engineering Humans Serum Albumin Bovine General Materials Science General Chemistry General Medicine CRISPR-Cas Systems Endonucleases Clathrin
Zdroj:	Journal of Materials Chemistry B. 10:7634-7649
ISSN:	2050-7518 2050-750X
DOI:	10.1039/d2tb00645f
Popis:	sgRNA/Cas9 ribonucleoproteins (RNPs) provide a site-specific robust gene-editing approach avoiding the mutagenesis and unwanted off-target effects. However, the high molecular weight (∼165 kDa), hydrophilicity and net supranegative charge (∼-20 mV) hinder the intracellular delivery of these RNPs. In the present study, we have prepared cationic RNPs lipopolymeric nanoplexes that showed a size of 117.3 ± 7.64 nm with +6.17 ± 1.04 mV zeta potential and90% entrapment efficiency of RNPs. Further, these RNPs lipopolymeric nanoplexes showed good complexation efficiency and were found to be stable for 12 h with fetal bovine serum. These RNPs lipopolymeric nanoplexes did not induce any significant cytotoxicity in HEK293T cells, and were efficiently uptaken
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c3cc1056c56e0bbb2c14e482f905d6e https://doi.org/10.1039/d2tb00645f Zobrazit plný text záznamu