On-Demand Regulation of Dual Thermosensitive Protein Hydrogels
| Autor: | Hai-Feng Chen, Xiao-Xia Xia, David L. Kaplan, Zhi-Gang Qian, Wenwen Song, Hao Liu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Polymers and Plastics Polymers Organic Chemistry Hydrogels Nanotechnology 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Dual (category theory) Inorganic Chemistry On demand Self-healing hydrogels Materials Chemistry Transition Temperature Peptides 0210 nano-technology Hydrophobic and Hydrophilic Interactions |
| Zdroj: | ACS Macro Letters. 10:395-400 |
| ISSN: | 2161-1653 |
| DOI: | 10.1021/acsmacrolett.1c00062 |
| Popis: | Despite considerable progress having been made in thermosensitive protein hydrogels, regulating their thermal transitions remains a challenge due to the intricate molecular structures and interactions of the underlying protein polymers. Here we report a genetic fusion strategy to tune the unique dual thermal transitions of the C-terminal domain (CTD) of spider major ampullate spidroin 1, and explore the regulation mechanism by biophysical characterization and molecular dynamics simulations. We found that the fusion of elastin-like polypeptides (ELPs) tuned the dual transition temperatures of CTD to a physiologically relevant window, by introducing extra hydrogen bonding at low temperatures and hydrophobic interactions at high temperatures. The resulting hydrogels constructed from the fusion proteins were demonstrated to be a promising vehicle for cell preservation and delivery. This study provides insights on the regulation of the dual thermosensitive protein hydrogels and suggests a potential application of the hydrogels for consolidated cell storage and delivery. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|