Comparative transcriptomics of Aspergillus fumigatus strains upon exposure to human airway epithelial cells
| Autor: | Scott G. Filler, Julie C. Dunning Hotopp, Tonya N. Watkins, Tracy H. Hazen, Matthew Chung, Vincent M. Bruno, Hong Liu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Spores
0301 basic medicine Respiratory System RNA-Seq Aspergillus fumigatus Af293 Transcriptome Pathogenesis Tissue culture Gene Expression Regulation Fungal 2.1 Biological and endogenous factors 2.2 Factors relating to the physical environment Lung biology General Medicine Spores Fungal CEA10 3. Good health Fungal Infectious Diseases Phenotype Host-Pathogen Interactions Respiratory Systems Microbiology: Transcriptomics Proteomics Networks Infection Sequence Analysis Biotechnology 1.1 Normal biological development and functioning 030106 microbiology Genes Fungal Microbiology 03 medical and health sciences Genetics Short Paper Humans Gene Epithelial Cells biology.organism_classification In vitro Emerging Infectious Diseases 030104 developmental biology Gene Expression Regulation Genes Cell culture A549 Cells Alveolar Epithelial Cells Pulmonary Aspergillosis RNA-seq airway epithelial cells |
| Zdroj: | Watkins, TN; Liu, H; Chung, M; Hazen, TH; Hotopp, JCD; Filler, SG; et al.(2018). Comparative transcriptomics of Aspergillus fumigatus strains upon exposure to human airway epithelial cells. MICROBIAL GENOMICS, 4(2). doi: 10.1099/mgen.0.000154. UCLA: Retrieved from: http://www.escholarship.org/uc/item/4271m3bp Microbial Genomics Microbial genomics, vol 4, iss 2 |
| DOI: | 10.1099/mgen.0.000154. |
| Popis: | Aspergillus fumigatus is an opportunistic, ubiquitous, saprophytic mould that can cause severe allergic responses in atopic individuals as well as life-threatening infections in immunocompromised patients. A critical step in the establishment of infection is the invasion of airway epithelial cells by the inhaled fungi. Understanding how A. fumigatus senses and responds to airway cells is important to understand the pathogenesis of invasive pulmonary aspergillosis. Here, we analysed the transcriptomes of two commonly used clinical isolates, Af293 and CEA10, during infection of the A549 type II pneumocyte cell line in vitro. We focused our RNA-seq analysis on the core set of genes that are present in the genomes of the two strains. Our results suggest that: (a) A. fumigatus does not mount a conserved transcriptional response to airway epithelial cells in our in vitro model and (b) strain background and time spent in the tissue culture media have a greater impact on the transcriptome than the presence of host cells. Our analyses reveal both common and strain-specific transcriptional programmes that allow for the generation of hypotheses about gene function as it pertains to pathogenesis and the significant phenotypic heterogeneity that is observed among A. fumigatus isolates. |
| Databáze: | OpenAIRE |
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