Expression of Escherichia coli heat-labile enterotoxin b subunit (LTB) in carrot (Daucus carota L.)
| Autor: | Sergio Rosales-Mendoza, Ruth Elena Soria-Guerra, Juan Pablo Loyola-Rodríguez, Rubén López-Revilla, Gerardo R. Argüello-Astorga, Ángel G. Alpuche-Solís, Maria Teresa Olivera-Flores, Ramón Fernando García-de la Cruz, Juan Francisco Jiménez-Bremont |
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| Rok vydání: | 2007 |
| Předmět: |
Escherichia coli Proteins
Protein subunit Bacterial Toxins Plant Science General Medicine Enterotoxin Biology Heat-labile enterotoxin Plants Genetically Modified medicine.disease_cause Daucus carota Microbiology law.invention Enterotoxins Transformation (genetics) Gene Expression Regulation Plant law Enterotoxigenic Escherichia coli Gene expression Escherichia coli Recombinant DNA medicine Agronomy and Crop Science |
| Zdroj: | Plant Cell Reports. 26:969-976 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-007-0310-2 |
| Popis: | We expressed the B subunit of enterotoxigenic Escherichia coli heat-labile enterotoxin (LTB) encoded by a synthetic codon-optimized gene in carrot. An Agrobacterium-mediated transformation method was used. Thirty independent transgenic lines were regenerated via somatic embryogenesis after 6 months in culture and were transferred to a greenhouse. GM1-ELISA assay was used to assess LTB protein content in mature taproots. Some transgenic lines expressed LTB up to 0.3% of the total soluble protein, which is tenfold higher than the expression levels reported earlier using the native bacterial gene in plants. Immunological assay confirmed proper assembly of the pentameric complex and in vitro activity of the recombinant LTB protein, suggesting that it can be functional in prevention of diarrhea. |
| Databáze: | OpenAIRE |
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