Knockdown of TSPAN1 by RNA silencing and antisense technique inhibits proliferation and infiltration of human skin squamous carcinoma cells
| Autor: | Jianwei Zhu, Yu-xiang Lu, Jianli Wang, Hui Li, Yuan-Yuan Wu, Guei-lan Wang, Jin Qin, Jinsheng Tuo, Zhu Yuanyuan, Li Chen |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
0301 basic medicine
Cancer Research Skin Neoplasms Tetraspanins Biology 03 medical and health sciences 0302 clinical medicine RNA interference Cell Line Tumor Skin Squamous Cell Carcinoma Humans RNA Antisense RNA Small Interfering Cell Proliferation Gene knockdown Membrane Proteins General Medicine Transfection Molecular biology Antisense RNA Squamous carcinoma 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer cell Cancer research Carcinoma Squamous Cell RNA Interference A431 cells |
| Zdroj: | Tumori. 96(2) |
| ISSN: | 0300-8916 |
| Popis: | Aim To explore the function of TSPAN1 in squamous cell skin carcinoma by means of TSPAN1-specific siRNA and antisense oligonucleotide techniques. Methods pU6H1-GFP-siRNA TSPAN1 and pcDNA3.1 antisense TSPAN1 were constructed and transfected into squamous cell skin carcinoma cell line A431 cells to knock down TSPAN1 gene expression. The levels of TSPAN1 mRNA and protein expression were detected by semiquantitive RT-PCR and Western blot, respectively. The proliferation rates of A431 cells were determined by MTT assay and flow cytometry. Lastly, the migration and infiltration of A431 cells were determined by the Transwell migration assay. Results Transfection with either pU6H1-GFP-siRNA TSPAN1 or pcDNA3.1 antisense TSPAN1 led to an obvious reduction of expression levels of TSPAN1 mRNA and protein in A431 cells, respectively. The proliferation, migration and infiltration of A431 cancer cells were significantly inhibited at 48 hours after transfection of plasmids harboring TSPAN1 siRNA and antisense RNA. Conclusion The TSPAN1 gene might play a role in the proliferation of squamous cell carcinoma of the skin and be associated with cancer cell motility, implying a function of the gene in the development of skin cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|