Identification of expression quantitative trait loci (eQTLs) in human peripheral blood mononuclear cells (PBMCs) and shared with liver and brain
| Autor: | Fang-Fei Xie, Xin Lu, Peng-Fei Bing, Jian Wu, Yu-Fan Guo, Ke-Qin Zeng, Pei He, Mingjun Wang, Wei Xia, Fei-Yan Deng, Shu-Feng Lei, Lan Wang, Yonghong Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine Quantitative Trait Loci Mutation Missense Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Biochemistry Peripheral blood mononuclear cell Arthritis Rheumatoid 03 medical and health sciences Gene expression Humans SNP Gene Regulatory Networks Allele Molecular Biology Gene Cells Cultured Aged Genetics Gene Expression Profiling Brain RNA Cell Biology Middle Aged 030104 developmental biology Gene Expression Regulation Liver Expression quantitative trait loci Leukocytes Mononuclear Female Genome-Wide Association Study |
| Zdroj: | Journal of Cellular Biochemistry. 119:1659-1669 |
| ISSN: | 1097-4644 0730-2312 |
| Popis: | PBMCs are essential for immunity and involved in various diseases. To identify genetic variations contributing to PBMCs transcriptome-wide gene expression, we performed a genome-wide eQTL analysis by using genome-wide SNPs data and transcriptome-wide mRNA expression data. To assess whether there are common regulation patterns shared among different tissues/organs, public datasets were utilized to identify common eQTLs shared with PBMCs in lymphoblastoid, monocytes, liver, and brain. Allelic expression imbalance (AEI) assay was employed to validate representative eQTLs identified. We identified 443 cis- and 2386 trans-eSNPs (FDR |
| Databáze: | OpenAIRE |
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