Metastasis in pleural mesothelioma. Immunohistochemical markers for disseminated disease
| Autor: | S K Suvarna, P D Lumb |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Mesothelioma
medicine.medical_specialty Pathology Histology Pleural Neoplasms Biology Pathology and Forensic Medicine Metastasis Antigens CD Antigens Neoplasm Biopsy Biomarkers Tumor medicine Humans Disseminated disease Neoplasm Metastasis Lung medicine.diagnostic_test Antibodies Monoclonal Anatomical pathology General Medicine medicine.disease Immunohistochemistry Staining Ki-67 Antigen medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Antibodies Antinuclear Basigin |
| Zdroj: | Histopathology. 44:345-352 |
| ISSN: | 1365-2559 0309-0167 |
| DOI: | 10.1111/j.1365-2559.2004.01844.x |
| Popis: | Aims: To examine 13 cases of mesothelioma with metastases and compare these with 29 biopsy samples of patients without metastases. Metastatic disease was defined as tumour in which tumour appeared in a different cavity/tissue of the body and which showed no direct spread. Consequently, mediastinal nodal and parenchymal lung spread was excluded. Methods and results: Standard sections were prepared and stained according to the manufacturers' protocols. The antibodies used were MIB-1, nm23, Bcl-2, MMP-9, EMMPRIN (CD147) and α-catenin. Scoring employed a grading system (0/1/2/3), and was performed by two pathologists independently. The tissues revealed no significant staining differences for MIB-1, Bcl-2, MMP-9 or EMMPRIN, and therefore no linkage to metastatic potential was determined. α-Catenin showed a diminished level of expression in cases of metastatic mesothelioma (P = 0.024), possibly reflecting dimished catenin–cadherin binding and paralleling data from other tumours. nm23 showed greater staining in metastatic tumours when compared with the controls (P = 0.001). Intriguingly, the nm23 staining pattern was the reverse of that expected. This reversed pattern has been noted before in other tumours and therefore a biological prognostic event may exist for this antibody test and mesothelioma metastasis. Conclusion: There may be a place for nm23 and possibly α-catenin in immunohistochemical assessment of mesothelioma metastatic potential. However, MIB-1, Bcl-2, MMP-9 and EMMPRIN (CD147) do not show significant staining results. |
| Databáze: | OpenAIRE |
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