Nucleotide sequence of the right early region of Bacillus subtilis phage PZA completes the 19366-bp sequence of PZA genome. Comparison with the homologous sequence of phage phi 29

Autor: Zdeněk Hostomský, Václav Pačes, Pavel Urbánek, Čestmír Vlček
Rok vydání: 1986
Předmět:
Zdroj: Gene. 44(1)
ISSN: 0378-1119
Popis: We have sequenced the rightmost 2079 bp of the Bacillus subtilis phage PZA genome. This region encompasses the right early region. We compared it with the homologous region of phage φ29. Six open reading frames (ORFs) were found in this region of PZA and one of them was assigned to gene 17. Analysis of putative ribosome-binding sites and comparison with φ29 ORFs indicate that at least some of the remaining ORFs could encode proteins. Corresponding genes were not identified so far by genetic methods. Promoter candidates in the right early region of PZA were found and compared to φ29 promoters. The sequenced region together with previously determined sequences [Paces et al., Gene 38 (1985) 45–56 and 44 (1986) 107–114] completes the entire 19366-bp sequence of phage PZA genome.
Databáze: OpenAIRE