Liver X receptor preferentially activates de novo lipogenesis in human preadipocytes
| Autor: | Christian Darimont, Ornella Avanti, Patricia Leone-Vautravers, Vittorio Giusti, Robert Mansourian, Katherine Macé, Irène Zbinden |
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| Rok vydání: | 2005 |
| Předmět: |
Agonist
medicine.medical_specialty medicine.drug_class Cell Peroxisome proliferator-activated receptor Receptors Cytoplasmic and Nuclear Biology Biochemistry Internal medicine medicine Adipocytes Humans Liver X receptor Cells Cultured Triglycerides Liver X Receptors chemistry.chemical_classification Lipogenesis Stem Cells Cell Differentiation General Medicine Stromal vascular fraction Orphan Nuclear Receptors DNA-Binding Proteins PPAR gamma medicine.anatomical_structure Endocrinology chemistry Gene Expression Regulation Cell culture Adipogenesis lipids (amino acids peptides and proteins) Stromal Cells Biomarkers Transcription Factors |
| Zdroj: | Biochimie. 88(3-4) |
| ISSN: | 0300-9084 |
| Popis: | The liver X receptor (LXR) was demonstrated to play a key role in cholesterol metabolism in liver, intestine and macrophage. However, its function on the regulation of preadipocyte differentiation remains unclear since contradictory results were reported. The objective of the present study was to unravel the functionality of LXR in human preadipocytes. We show that the LXR agonist T0901317 strongly stimulated the expression of SREBP-1c and the lipogenic enzymes ACC-1, FAS and SCD-1 in both the human preadipose cell line Chub-S7 as well as human primary stromal vascular fraction (SVF) cells. The effects on gene expression were associated with the stimulation of de novo lipogenesis in both cell models, resulting in the induction of lipid accumulation. In contrast with a PPARgamma agonist (BRL49653), T0901317 enhanced only slightly the expression of PPARgamma dependent genes (PPARgamma, aP2 and adiponectin) in Chub-S7 cells and failed to change their expression in human SVF cells. These results show that LXR stimulated preferentially triglyceride accumulation in human preadipocytes via the induction of de novo lipogenesis, rather than activating the differentiation process through PPARgamma activation. |
| Databáze: | OpenAIRE |
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