Identification of Atg8 from Acanthamoeba castellanii by genetic complementation in Saccharomyces cerevisiae
| Autor: | Christine Braquart-Varnier, Yann Héchard, Matthieu Régnacq, Pierre Voisin, Ascel Samba-Louaka, Thierry Bergès |
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| Přispěvatelé: | Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Microbiologie de l'Eau (MDE), Ecologie et biologie des interactions (EBI), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Ecologie, Evolution, Symbiose (EES), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
ATG8 030106 microbiology Saccharomyces cerevisiae Protozoan Proteins Gene Expression [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Genome 03 medical and health sciences Complementary DNA parasitic diseases Autophagy [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology Gene Genetics Acanthamoeba castellanii Genetic Complementation Test Autophagy-Related Protein 8 Family biology.organism_classification Cell biology Complementation 030104 developmental biology Parasitology Atg8 |
| Zdroj: | Molecular and Biochemical Parasitology Molecular and Biochemical Parasitology, Elsevier, 2016, 210 (1-2), pp.55-57. ⟨10.1016/j.molbiopara.2016.08.006⟩ |
| ISSN: | 0166-6851 |
| Popis: | International audience; Autophagy is a eukaryotic process responsible for the degradation of intracellular content such as damaged organelles. Several putative autophagy-related genes have been identified within the annotated genome of the free-living amoeba Acanthamoeba castellanii. However, the involvement of the corresponding proteins in the autophagy pathway had not been formerly established. Here, we report that AcAtg8 cDNA can complement ATG8-deficient Saccharomyces cerevisiae. |
| Databáze: | OpenAIRE |
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