In vitro comparison of three percutaneous atrial septal defect closure devices for endothelialisation and haemocompatibility

Autor: Zakaria Jalal, Audrey Aussel, Laurence Bordenave, Marlène Durand, J.B. Thambo, Yael Levy, Noélie B. Thebaud, Jean Ripoche, Reine Bareille, Martine Renard
Přispěvatelé: Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] (CRCTB), Université Bordeaux Segalen - Bordeaux 2-CHU Bordeaux [Bordeaux]-Institut National de la Santé et de la Recherche Médicale (INSERM), Bioingénierie tissulaire (BIOTIS), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), CIC Bordeaux, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), CCSD, Accord Elsevier
Rok vydání: 2020
Předmět:
Zdroj: Archives of cardiovascular diseases
Archives of cardiovascular diseases, Elsevier/French Society of Cardiology, 2020, 113, pp.503-512. ⟨10.1016/j.acvd.2020.03.022⟩
ISSN: 1875-2136
1875-2128
DOI: 10.1016/j.acvd.2020.03.022
Popis: Summary Background Percutaneous device closure of atrial septal defect (ASD) is the gold-standard treatment, but several delayed complications may occur as a result of incomplete device endothelialisation. Aims In this in vitro study, we compared three ASD closure devices [Nit-Occlud® ASD-R (device 1); Hyperion™ ASDO (device 2); and Amplatzer™ Septal Occluder (device 3)] in terms of the endothelialisation process, using human endothelial progenitors cells (EPCs), and haemocompatibility. Methods EPCs from umbilical cord blood were extracted, cultured and characterised. Device samples were seeded with 100,000 cells/cm2. EPC adhesion was investigated at 3 and 24 hours, and EPC proliferation was monitored, which allowed longitudinal follow-up (days 1–12). Haemocompatibility of device samples was assessed using a complement C3a assay and platelet and coagulation activation. Results With regard to EPC adhesion and proliferation, no statistically significant differences were found between the three devices. We observed for each device a significant time-dependent EPC proliferation, appearing at day 8 for devices 2 and 3 and day 10 for device 1. No complement or platelet activation occurred within 15 minutes of contact with devices. However, there was minimal activation of coagulation for the three devices. Conclusions In this in vitro study we showed that, despite the three ASD occluders having different device designs and coatings, adhesion and proliferation of human endothelial cells was similar for all devices. This should be further confirmed by similar studies including shear stress forces and anti-thrombotic treatments.
Databáze: OpenAIRE
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