In vitro polyembryony induction in a critically endangered fern, Pteris tripartita Sw
| Autor: | Baskaran Xavier Ravi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Polyembryony lcsh:Medicine Pteris tripartita Plant Science Biology 010603 evolutionary biology 01 natural sciences 6-Benzylaminopurine chemistry.chemical_compound Murashige and Skoog medium Gametophyte Botany Spore germination General Veterinary Murashige and Skoog basal media lcsh:R Obstetrics and Gynecology Sporophyte Spore Reproductive Medicine chemistry Animal Science and Zoology Juvenile sporophytes 010606 plant biology & botany |
| Zdroj: | Asian Pacific Journal of Reproduction, Vol 5, Iss 4, Pp 345-350 (2016) |
| ISSN: | 2305-0500 |
| DOI: | 10.1016/j.apjr.2016.06.012 |
| Popis: | Objective An efficient in vitro protocol was established during the development of polyembryony in the spore derived gametophyte of Pteris tripartita Sw. Methods Sterilized spores were germinated in half strength Murashige and Skoog (MS) basal media to produce gametophyte at in vitro condition. Three-month-old gametophytes were sub-cultured in half strength MS medium with 6-benzylaminopurine (BAP). All inoculated cultures were incubated and noticed every month for polyembryony. Results Multiple numbers (Di, Tri, Tetra, Hexa, and Octa) of the juvenile sporophyte per spore derived gametophyte were observed at 3 mg/L of BAP in half strength MS basal media, which augmented with 30% sucrose. Hexa polyembryony or juvenile sporophytes was noticed on both 3 and 4 mg/L of BAP, which developed 1.00 and 0.76 cm lengths, respectively. At 4 mg/L of BAP in MS culture medium, various numbers (Di, Tri, Tetra and Hexa) of juvenile sporophytes per gametophyte were formed and showed 1.03, 0.66, 0.53 and 0.76 cm of sporophyte lengths, correspondingly. Conclusion An in vitro developed polyembryony or juvenile sporophyte exhibited normal growth in their morphological structure. |
| Databáze: | OpenAIRE |
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