Muscle-specific androgen receptor deletion shows limited actions in myoblasts but not in myofibers in different muscles in vivo
| Autor: | Jarrod P Skinner, Maria Ws Chiu, Barbara C Fam, Nicole K. L. Lee, Kesha Rana, Patricia K Russell, Jeffrey D Zajac, Helen E. MacLean |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Anabolism medicine.drug_class Biology Myoblasts Mice 03 medical and health sciences Endocrinology Myofibrils Physical Conditioning Animal medicine Animals Humans Myocyte Receptor Molecular Biology Mice Knockout Muscles Skeletal muscle Organ Size Androgen Cell biology Androgen receptor 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Myogenic Regulatory Factors Organ Specificity Receptors Androgen Myogenic regulatory factors Myofibril Biomarkers Gene Deletion |
| Zdroj: | Journal of Molecular Endocrinology. 57:125-138 |
| ISSN: | 1479-6813 0952-5041 |
| DOI: | 10.1530/jme-15-0320 |
| Popis: | The aim of this study was to investigate the direct muscle cell-mediated actions of androgens by comparing two different mouse lines. The cre-loxP system was used to delete the DNA-binding activity of the androgen receptor (AR) in mature myofibers (MCK mARΔZF2) in one model and the DNA-binding activity of the AR in both proliferating myoblasts and myofibers (α-actin mARΔZF2) in another model. We found that hind-limb muscle mass was normal in MCK mARΔZF2mice and that relative mass of only some hind-limb muscles was reduced in α-actin mARΔZF2mice. This suggests that myoblasts and myofibers are not the major cellular targets mediating the anabolic actions of androgens on male muscle during growth and development. Levator ani muscle mass was decreased in both mouse lines, demonstrating that there is a myofiber-specific effect in this unique androgen-dependent muscle. We found that the pattern of expression of genes includingc-myc,Fzd4andIgf2is associated with androgen-dependent changes in muscle mass; therefore, these genes are likely to be mediators of anabolic actions of androgens. Further research is required to identify the major targets of androgen actions in muscle, which are likely to include indirect actions via other tissues. |
| Databáze: | OpenAIRE |
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