Gap junction uncoupling of neonatal rat astroglial cultures does not affect their expression of fibroblast growth factor 2
| Autor: | A. Heinke, Klaus Unsicker, B. Reuss |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Microinjections Blotting Western Connexin Gene Expression Biology Fibroblast growth factor Cell junction chemistry.chemical_compound Mesencephalon Internal medicine medicine Animals RNA Messenger Cells Cultured Cerebral Cortex Lucifer yellow Arachidonic Acid Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Gap junction Gap Junctions Isoquinolines Corpus Striatum Cell biology Rats medicine.anatomical_structure Endocrinology chemistry Animals Newborn Astrocytes Neuroglia Glycyrrhetinic Acid Fibroblast Growth Factor 2 Intracellular Astrocyte |
| Zdroj: | Neuroscience letters. 258(1) |
| ISSN: | 0304-3940 |
| Popis: | Fibroblast growth factor (FGF)-2 is expressed by astroglial cells and regulates astroglial proliferation, differentiation, and gap junction communication. We show now that uncoupling of astroglial cells cultured from neonatal rat cortex, striatum and mesencephalon by 18alpha-glycirrhetinic acid (AGA) or arachidonic acid (AA) (demonstrated by intracellular injections of Lucifer Yellow) does affect neither levels of FGF-2 mRNA (investigated by a competitive RT-PCR method), nor protein (as revealed by Western blot analysis). This suggests that expression of FGF-2, which reduces gap junction communication of cortical and striatal astroglial cells [15], is not affected when astroglial cells are uncoupled. Regulation of FGF-2 expression and coupling in astroglial cells are thus not reciprocal suggesting more complex interrelationships between these two important parameters of astroglial cell differentiation. |
| Databáze: | OpenAIRE |
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