Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p
| Autor: | Sylvie Turcotte, Maryse Thivierge, Jana Stankova, Marek Rola-Pleszczynski |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Article Subject Receptor expression T cell Immunology Lymphocyte Activation Peripheral blood mononuclear cell Arthropod Proteins 03 medical and health sciences Interleukin 21 0302 clinical medicine T-Lymphocyte Subsets medicine Hypersensitivity Immunology and Allergy Cytotoxic T cell Animals Humans Antigens Dermatophagoides Receptor Cells Cultured 030304 developmental biology House dust mite Receptors Leukotriene 0303 health sciences biology Pyroglyphidae General Medicine respiratory system biology.organism_classification Cysteine Endopeptidases medicine.anatomical_structure Gene Expression Regulation lcsh:RC581-607 CD8 Biomarkers 030215 immunology Research Article |
| Zdroj: | Journal of Immunology Research, Vol 2015 (2015) Journal of Immunology Research |
| ISSN: | 2314-7156 2314-8861 |
| Popis: | In order to determine the potential for allergen to modulate T cell expression of the CysLT1receptor and responsiveness to leukotrienes, peripheral blood mononuclear cells from house dust mite-allergic or nonallergic individuals were incubated withD. pteronyssinusallergen (Der p). Baseline CysLT1expression was similar in both groups of donors, but Der p significantly enhanced CysLT1expression in CD4+and CD8+T cells of only allergic individuals and induced enhanced responsiveness of CD4+T cells to LTD4in terms of calcium mobilisation. This effect was prevented by the CysLT1antagonist MK571. Der p also induced IL-4 and IL-10 production, and neutralizing antibody to IL-4 prevented both the enhanced CysLT1expression and the enhanced responsiveness of T cells to LTD4induced by Der p. In allergic individuals, Der p also induced T cell proliferation and a Th2-biased phenotype. Our data suggest that, in allergen-sensitized individuals, exposure to allergen can enhance T cell expression of CysLT1receptors through a mechanism involving IL-4 production. This, in turn, would induce CD4+T cell responsiveness to cysteinyl-leukotrienes and Th2 cell activation. |
| Databáze: | OpenAIRE |
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