Japanese quail: an efficient animal model for the production of transgenic avians
| Autor: | Greg Poynter, David Huss, Rusty Lansford |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
animal structures
Transgene ved/biology.organism_classification_rank.species Coturnix In ovo General Biochemistry Genetics and Molecular Biology Animals Genetically Modified Birds Mice biology.animal Animals Humans Transgenes Model organism Promoter Regions Genetic Gene knockout Fluorescent Dyes Genetics biology business.industry ved/biology Lentivirus Genomics biology.organism_classification Quail Biotechnology Genetically modified organism Rats Genetic Techniques embryonic structures Models Animal business Developmental biology Developmental Biology |
| Zdroj: | Cold Spring Harbor protocols. 2009(1) |
| ISSN: | 1559-6095 |
| Popis: | INTRODUCTIONThe ability to generate transgenic mice has been a powerful tool in studying functional genomics, and much of our knowledge about developmental biology has come from the study of chicken embryology. Unfortunately, the availability of molecular genetic techniques, such as transgenics and knockouts, has been limited for developmental biologists using avian animal models. Efforts to develop a system for the rapid production of transgenic chickens have met with many obstacles, including high animal husbandry costs and long generational times. Recently, the Japanese quail has proven to be an excellent model organism for the production of transgenic avians using lentiviral vectors. The relatively small size of the adults, short time to sexual maturity, and prodigious egg production of the Japanese quail make development of transgenic lines less labor- and space-intensive compared to chickens. The high degree of homology between chicken and quail genomes allows researchers to design highly specific DNA constructs for the production of transgenic birds. In addition, transgenic quail offer all of the advantages of the classic avian developmental model system, such as the ability to readily produce quail:chick transplant chimeras. Finally, Japanese quail are ideal for in ovo imaging of embryos expressing fluorescent reporters introduced from a transgene and/or electroporation. Here, we provide detailed methods for generating transgenic quail using high-titer lentivirus. |
| Databáze: | OpenAIRE |
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