Detection of CTX-M-15-Producing Escherichia coli Isolates of Lineages ST131-B2 and ST167-A in Environmental Samples of a Tunisian Hospital

Autor: Carla Andrea Alonso, Raoudha Dziri, Abdellatif Boudabous, Leila Ben Said, Karim Ben Slama, Carmen Torres, Ridha Bellaaj, Sarra Chairat, Ahlem Jouini, Naouel Klibi
Přispěvatelé: Université de Tunis - El Manar II, Université de Tunis El Manar (UTM), Universidad de La Rioja (UR), Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire (LR11IPT03), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Hôpital Militaire de Tunis HMPIT, This work was partially supported by the Tunisian Ministry of Higher Education and Scientific Research and by the Ministerio de Economía y Competitividad (MINECO) of Spain (project SAF2012-35474), and Fondo Europeo de Desarrollo Regional (FEDER). C.A.A. has a predoctoral fellowship of MINECO associated to project SAF2012-35474.
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
MESH: Sequence Analysis
DNA
[SDV]Life Sciences [q-bio]
Gene Expression
MESH: beta-Lactamases
medicine.disease_cause
Polymerase Chain Reaction
law.invention
Integrons
law
Gene expression
Medicine
MESH: Phylogeny
Polymerase chain reaction
Escherichia coli Infections
Phylogeny
Skin
Cross Infection
MESH: Microbial Sensitivity Tests
MESH: Escherichia coli
MESH: Hospitals
Hospitals
3. Good health
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
MESH: Integrons
Gene cassette
MESH: Electrophoresis
Gel
Pulsed-Field
Fomites
Female
MESH: Tunisia
Plasmids
Microbiology (medical)
Tunisia
MESH: Gene Expression
Sequence analysis
Virulence Factors
030106 microbiology
Immunology
Microbial Sensitivity Tests
beta-Lactams
Microbiology
beta-Lactam Resistance
beta-Lactamases
03 medical and health sciences
MESH: Skin
Phylogenetics
MESH: Plasmids
MESH: beta-Lactams
MESH: Anti-Bacterial Agents
Escherichia coli
Humans
Gene
MESH: Virulence Factors
MESH: Escherichia coli Infections
Pharmacology
MESH: Humans
business.industry
MESH: Cross Infection
MESH: Polymerase Chain Reaction
Sequence Analysis
DNA
Sequence types
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
MESH: Fomites
MESH: Male
MESH: beta-Lactam Resistance
business
MESH: Female
Zdroj: Microbial Drug Resistance
Microbial Drug Resistance, Mary Ann Liebert, 2016, 22 (5), pp.399-403. ⟨10.1089/mdr.2015.0354⟩
ISSN: 1076-6294
DOI: 10.1089/mdr.2015.0354⟩
Popis: International audience; To investigate the possible role of the hospital environment in the dissemination of extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli isolates, 300 samples were taken during 2013 from abiotic surfaces (n = 250), healthcare worker hands (n = 27), and hands of patients (n = 23) in a Tunisian Hospital. ESBL-producing E. coli isolates were recovered in 3.7% of analyzed samples (4% abiotic surfaces; 4.3% hands of patients; 0% in healthcare worker hands), and one isolate/sample was further studied. The characterization of beta-lactamase genes, as well as the genetic environment of bla CTX-M gene, was performed by PCR and sequencing. The ESBL genes found were as follows: bla CTX-M-15 (eight isolates), bla CTX-M-15 +bla SHV-12 (two isolates), and bla SHV-12 (one isolate). The bla TEM-1b gene was detected in seven ESBL-positive isolates. The orf477 was found downstream of bla CTX-M-15 gene in 10 strains, whereas the ISEcp1 sequence was identified upstream of this gene in two isolates. The analysis of class 1 integrons by PCR and sequencing revealed five positive isolates with the following gene cassette arrangements: dfrA1-aadA1 (two isolates), aadA1 (two isolates), and aadA2 (one isolate). The virulence-encoding genes aer, eae, bfp, and hly were detected by PCR in six, four, four, and three isolates, respectively. The following sequence types and associated phylogroups were detected among ESBL-producing strains: ST167-phylogroup-A (six isolates) and ST131-phylogroup-B2 (two isolates). In conclusion, the hospital environment could be a reservoir of multiresistant bacteria, including ESBL-positive E. coli isolates, which could be acquired by the patient population, and strict control measures should be established to minimize this problem.
Databáze: OpenAIRE
Externí odkaz: