Zebrafish provide a sensitive model of persisting neurobehavioral effects of developmental chlorpyrifos exposure: Comparison with nicotine and pilocarpine effects and relationship to dopamine deficits
| Autor: | Donnie Eddins, Elwood Linney, Paul Williams, Daniel T. Cerutti, Edward D. Levin |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Insecticides
Nicotine Reflex Startle Serotonin medicine.medical_specialty Startle response Time Factors Dopamine Muscarinic Agonists Toxicology Article Cellular and Molecular Neuroscience chemistry.chemical_compound Developmental Neuroscience Internal medicine Toxicity Tests medicine Animals Nicotinic Agonists Habituation Habituation Psychophysiologic Neurotransmitter Zebrafish medicine.diagnostic_test Organophosphate Pilocarpine Neurotoxicity Brain medicine.disease Endocrinology Nicotinic agonist chemistry Maternal Exposure Larva Models Animal Female Chlorpyrifos Psychology medicine.drug |
| Zdroj: | Neurotoxicology and Teratology. 32:99-108 |
| ISSN: | 0892-0362 |
| DOI: | 10.1016/j.ntt.2009.02.005 |
| Popis: | Chlorpyrifos (CPF) an organophosphate pesticide causes persisting behavioral dysfunction in rat models when exposure is during early development. In earlier work zebrafish were used as a complementary model to study mechanisms of CPF-induced neurotoxicity induced during early development. We found that developmental (first five days after fertilization) chlorpyrifos exposure significantly impaired learning in zebrafish. However, this testing was time and labor intensive. In the current study we tested the hypothesis that persisting effects of developmental chlorpyrifos could be detected with a brief automated assessment of startle response and that this behavioral index could be used to help determine the neurobehavioral mechanisms for persisting CPF effects. The swimming activity of adult zebrafish was assessed by a computerized video-tracking device after a sudden tap to the test arena. Ten consecutive trials (1/min) were run to determine startle response and its habituation. Additionally, habituation recovery trials were run at 8, 32 and 128 min after the end of the initial trial set. CPF-exposed fish showed a significantly (p |
| Databáze: | OpenAIRE |
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