Polymorphism of the thrombostasin gene in the horn fly (Haematobia irritans) revealed in a cDNA library and in genomic DNA
| Autor: | Dunhua Zhang, Cupp Ew, Cupp Ms |
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| Rok vydání: | 2001 |
| Předmět: |
Male
DNA Complementary Molecular Sequence Data medicine.disease_cause Genome Sequence Homology Nucleic Acid Genetics medicine Animals Protein Isoforms Amino Acid Sequence Allele Molecular Biology Gene Gene Library Messenger RNA Mutation Polymorphism Genetic Base Sequence Sequence Homology Amino Acid biology cDNA library Muscidae fungi DNA Sequence Analysis DNA General Medicine biology.organism_classification Molecular biology Haematobia irritans genomic DNA Insect Proteins Female Sequence Alignment |
| Zdroj: | Molecular Genetics and Genomics. 266:296-302 |
| ISSN: | 1617-4623 1617-4615 |
| DOI: | 10.1007/s004380100556 |
| Popis: | Thrombostasin (TS) is a newly described thrombin-inhibiting protein isolated from the saliva of the horn fly (Haematobia irritans), a blood-sucking ectoparasite of cattle. This report provides a detailed characterization of the TS gene and the first analysis of the allelic complexity of a gene for an anti-hemostatic protein from a blood-feeding insect. Multiple point mutations at fixed positions in the TS gene were identified in a cDNA library prepared from mRNA isolated from horn fly salivary glands. When translated, the variant mRNAs would specify five biochemically active peptides that differ in molecular weight, isoelectric point and predicted secondary structure. Allelic variation with the same mutation pattern was revealed in the genomes of individual flies collected in the field and sampled from a long-standing laboratory colony. Approximately 60% of flies examined carried heterozygous alleles, including five additional alleles not found in the cDNA library. Comparative analysis of the allelic mutations and the predicted effects on secondary structures of the active proteins produced suggest that the TS gene may be undergoing evolutionary selection. |
| Databáze: | OpenAIRE |
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