Modulation of endogenous prostaglandins by thymosin-alpha 1 in lymphocytes
| Autor: | T Jezzi, Cartesio Favalli, Allan L. Goldstein, Cristina Rinaldi-Garaci, Vera Del Gobbo, Enrico Garaci |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
medicine.medical_specialty Rosette Formation Thymalfasin medicine.medical_treatment Lymphocyte Immunology Indomethacin Spleen Stimulation Biology Dinoprostone Mice Internal medicine medicine Splenocyte Animals Lymphocytes Prostaglandin E2 Prostaglandins E Thymosin Thymus Hormones Thymocyte Endocrinology medicine.anatomical_structure medicine.drug Prostaglandin E |
| Zdroj: | Cellular immunology. 80(1) |
| ISSN: | 0008-8749 |
| Popis: | The effects of thymosin- α 1 on the stimulation of specific release of prostaglandin E 2 (PGE 2 ) from splenic lymphocytes and thymocytes were studied. Experiments were also performed to study in parallel the absolute levels of thymosin- α 1 in the blood and the induction of serum FTS activity and of azathioprine sensitivity of spleen cells from adult thymectomized (ATx) mice. A significant difference in the release of PGE 2 between normal splenocytes and splenocytes from ATx mice was observed. Thymosin- α 1 at certain concentrations was able to stimulate PGE 2 release from lymphocytes of ATx mice while inhibiting release in lymphocytes of normal mice. Also, thymocytes were stimulated to release PGE 2 after incubation with α 1 in a manner similar to that seen in spleen cells of ATx mice. Approximately the same concentration of α 1 was found to also correct the low azathioprine sensitivity of splenocytes from ATx mice. Determinations of FTS-like activity in the blood and the pharmacokinetics of α 1 after administration of this synthetic molecule show a clear dissociation. A maximum peak of α 1 activity was obtained after 1 hr, while maximal FTS-like activity was observed after 24 hr. The inhibition of the induction by α 1 of FTS-like activity and of Thy 1.2 antigen by indomethacin suggests that the action of α 1 requires prostaglandin biosynthesis. |
| Databáze: | OpenAIRE |
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