Distinct bacterial community structure of 3 tropical volcanic soils from banana plantations contaminated with chlordecone in Guadeloupe (French West Indies)

Autor: Anne Mercier, Dominique Breeze, Jennifer Harris-Hellal, Marie-Christine Dictor, Christophe Mouvet
Přispěvatelé: Bureau de Recherches Géologiques et Minières (BRGM) (BRGM), Biogéochimie environnementale, French Ministry of Environment (contract 2010 SU 0006693)
Rok vydání: 2013
Předmět:
Insecticides
Environmental Engineering
Environmental remediation
[SDE.MCG]Environmental Sciences/Global Changes
Health
Toxicology and Mutagenesis
010501 environmental sciences
DNA
Ribosomal
complex mixtures
01 natural sciences
Volcanic soil
03 medical and health sciences
French West Indies
Soil Pollutants
Environmental Chemistry
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Biomass
Guadeloupe
DNA extraction
Soil Microbiology
030304 developmental biology
0105 earth and related environmental sciences
2. Zero hunger
0303 health sciences
Biomass (ecology)
geography
geography.geographical_feature_category
Bacteria
Ecology
Pesticide Residues
Public Health
Environmental and Occupational Health
Community structure
Agriculture
Musa
Biodiversity
General Medicine
General Chemistry
Contamination
Pollution
6. Clean water
Andosol
Volcano
Chlordecone
13. Climate action
Soil water
Bacterial community structure
Environmental science
Nitisol
Polymorphism
Restriction Fragment Length
Zdroj: Chemosphere
Chemosphere, Elsevier, 2013, 92 (7), pp.787-794. ⟨10.1016/j.chemosphere.2013.04.016⟩
ISSN: 0045-6535
Popis: International audience; In the French West Indies (FWI), the soil, andosols, ferralsols and nitisols, is highly polluted by chlordecone, although this organochlorine insecticide extensively applied to banana crops has been banned for 20 years. This contamination has led to a major human health concern inducing the need for remediation of the contaminated soils. Work was conducted to help to evaluate the impact of remediation processes on the microbial communities from these soils. Microbial biomass was estimated after direct DNA extraction from three chlordecone-contaminated soils (an andosol, a ferralsol and a nitisol) and the bacterial community analyzed using t-RFLP. The FWI volcanic andosol was particularly recalcitrant to usual direct DNA extraction protocols hampering analysis of soil microbial communities until now, in contrast with the 2 other soils. For the first time, DNA was directly extracted from a FWI andosol based on yeast RNA addition at the lysis step. Differences in microbial biomass were thus observed between the 3 FWI soils. Moreover, the bacterial community structure was significantly distinct from each other's and related to soil physico-chemical characteristics. Interestingly, differences in bacterial diversity could not be exclusively attributed to the level of chlordecone contamination.
Databáze: OpenAIRE
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