Determination of hydroxylated fatty acids from the biopolymer of tomato cutin and their fate during incubation in soil
| Autor: | Michal Shechter, Walter Vetter, Benny Chefetz, Simone Hauff |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Trimethylsilyl Compounds Cuticle Plant Science Cutin engineering.material Hydroxylation Biochemistry Gas Chromatography-Mass Spectrometry Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Membrane Lipids Soil Biopolymers Solanum lycopersicum Drug Discovery Organic chemistry Incubation Chemistry Fatty Acids food and beverages Esters General Medicine Biodegradation Biodegradation Environmental Complementary and alternative medicine Plant cuticle engineering Molecular Medicine Indicators and Reagents Biopolymer Hydrogenation Gas chromatography–mass spectrometry Food Science |
| Zdroj: | Phytochemical analysis : PCA. 21(6) |
| ISSN: | 1099-1565 |
| Popis: | Introduction – The plant cuticle is a thin, predominantly lipid layer that covers all primary aerial surfaces of vascular plants. The monomeric building blocks of the cutin biopolymer are mainly ω-hydroxy fatty acids. Objective – Analysis of ω-hydroxy fatty acids from cutin isolated from tomato fruits at different stages of decomposition in soil. Different derivatives and mass spectrometric techniques were used for peak identification and evaluation. Methodology – Preparation of purified cutin involving dewaxing and HCl treatment. Incubation of purified cutin for 20 months in soil. Pentafluorobenzoyl derivatives were used for GC/MS operated in the electron capture negative ion (ECNI) mode and trimethylsilyl ethers for GC/MS operated in the electron ionisation (EI) mode for analysis of ω-hydroxy fatty acids. Results – Six ω-hydroxy fatty acids were detected in the purified cutin, three of which were identified as degradation products of 9,16-dihydroxyhexadecanoic acid as a consequence of the HCl treatment involved in the purification step. Incubation of the isolated cutin in soil was accompanied with decrease in concentration of all hydroxyl fatty acids. Conclusion – We produced evidence that the HCl treatment only affected free hydroxyl groups and thus could be used for proportioning free and bound OH-groups on cutin fatty acids. The method enabled a direct quantification of the ω-hydroxy fatty acids throughout the incubation phase. Copyright © 2010 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text