Broadening the H5N3 Vaccine Immunogenicity against H5N1 Virus by Modification of Neutralizing Epitopes

Autor: Sharenya Chelvaretnam, Subaschandrabose Rajesh Kumar, Mookkan Prabakaran, Yunrui Tan
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Influenzavirus A
0301 basic medicine
Cross Protection
viruses
030106 microbiology
lcsh:QR1-502
Hemagglutinin (influenza)
Hemagglutinin Glycoproteins
Influenza Virus
Cross immunity
Antibodies
Viral
medicine.disease_cause
highly pathogenic avian influenza H5N1 (HPAI-H5N1)
Epitope
Virus
lcsh:Microbiology
Madin Darby Canine Kidney Cells
Epitopes
Mice
03 medical and health sciences
Dogs
Immunogenicity
Vaccine
Virology
Reassortant Viruses
medicine
neutralizing epitopes
Animals
antibody-guided design
Mice
Inbred BALB C
Influenza A Virus
H5N1 Subtype
biology
Communication
virus diseases
epitope modified vaccine
Antibodies
Neutralizing
Influenza A virus subtype H5N1
Vaccination
030104 developmental biology
Infectious Diseases
Amino Acid Substitution
Vaccines
Inactivated
Influenza Vaccines
Influenza in Birds
biology.protein
Female
Antibody
Chickens
Zdroj: Viruses, Vol 10, Iss 1, p 2 (2017)
Viruses
ISSN: 1999-4915
Popis: The highly pathogenic avian influenza (HPAI) H5N1 virus remains to be one of the world’s largest pandemic threats due to the emergence of new variants. The rapid evolution of new sub-lineages is currently the greatest challenge in vaccine development. In this study, we developed an epitope modified non-pathogenic H5N3 (A/duck/Singapore/97) vaccine for broad protection against influenza H5 subtype. H5N3 hemagglutinin (HA) mutant reassortant viruses with A/Puerto Rico/8/34 (PR8) backbone were generated by mutating amino acids at the 140th loop and 190th α-helix of hemagglutinin. The cross-neutralizing efficacy of reverse genetics-derived H5N3HA (RG-H5N3HA) mutants was confirmed by testing reactivity with reference chicken anti-H5N1 clade 2 virus sera. Furthermore, RG-H5N3HA mutant immunized mice induced cross-neutralizing antibodies and cross-protection against distinct H5N1 viral infection. Our findings suggest that the use of non-pathogenic H5 viruses antigenically related to HPAI-H5N1 allows for the development of broadly protective vaccines and reduces the need for biosafety level 3 (BSL3) containment facilities.
Databáze: OpenAIRE
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