miR-22-3p Suppresses Endothelial Progenitor Cell Proliferation and Migration via Inhibiting Onecut 1 (OC1)/Vascular Endothelial Growth Factor A (VEGFA) Signaling Pathway and Its Clinical Significance in Venous Thrombosis
Autor: | Zhibing Ming, Huoqi Liang, Yibiao Chen, Hefei Li, Xiaoqiang Yu, Chongjun Zhong, Chunqiu Xia |
---|---|
Rok vydání: | 2020 |
Předmět: |
Adult
Vascular Endothelial Growth Factor A 030204 cardiovascular system & hematology Endothelial progenitor cell 03 medical and health sciences 0302 clinical medicine Western blot Downregulation and upregulation Cell Movement Clinical Research medicine Humans Progenitor cell Cell Proliferation Endothelial Progenitor Cells Venous Thrombosis medicine.diagnostic_test business.industry Regeneration (biology) Stem Cells General Medicine Prognosis Endothelial stem cell Vascular endothelial growth factor A MicroRNAs 030220 oncology & carcinogenesis Case-Control Studies Cancer research Signal transduction business Onecut Transcription Factors Signal Transduction |
Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
ISSN: | 1643-3750 |
Popis: | BACKGROUND Proliferation and migration play crucial roles in various physiological processes, especially in injured endothelial repair. Endothelial progenitor cells (EPCs), as the precursors of endothelial cell, are involved in the regeneration of the endothelial lining of blood vessels. Furthermore, EPCs were found to be a potential choice for venous thrombosis (VT) treatment. MATERIAL AND METHODS EPCs were isolated from human peripheral blood of healthy adults and VT patients. Differently expressed micro(mi)RNAs were examined by quantitative real-time polymerase chain reaction, after which proliferative capacity and migration effect were tested by Cell-Counting Kit 8, scratch wound assay, and transwell assays. Bioinformatic analysis was applied to investigate the potential target messenger ribonucleic acid and a dual-luciferase reporting system was utilized to confirm the binding of miR-22-3p to its target gene. Western blot was carried out to detect candidate protein expression level. Finally, miR-22-3p expression was monitored in VT patients during follow-up to assess its correlation with prognosis of VT. RESULTS Our data revealed that miR-22-3p was upregulated in EPCs derived from deep VT (DVT) individuals and suppression of miR-22-3p contributed to proliferation and migration of EPCs. In addition, miR-22-3p/onecut 1 (OC1)/vascular endothelial growth factor A (VEGFA) signaling pathway was involved in regulating EPC migration and proliferation. In addition, lower expression of miR-22-3p in DVT patients indicated decreased risk of VT recurrence. CONCLUSIONS Our results suggest that miR-22-3p regulates OC1/VEGFA signaling and is involved in regulating EPC proliferation and migration. The expression level of miR-22-3p could be monitored to predict DVT patients' prognosis. |
Databáze: | OpenAIRE |
Externí odkaz: |
načítá se...