Aerobic metabolism on muscle contraction in porcine iris sphincter
| Autor: | Kazumasa Shimizu, Takuya Yogo, Takeharu Kaneda, Asami Kato, Norimoto Urakawa, Yasusi Hara, Hidenori Kanda, Yasuji Harada |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
030110 physiology 0301 basic medicine medicine.medical_specialty Carbachol Contraction (grammar) Phosphocreatine Swine 040301 veterinary sciences Iris Creatine Potassium Chloride 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate Oxygen Consumption Sodium Cyanide Internal medicine medicine Animals Sodium cyanide Pharmacology Full Paper General Veterinary hypoxia aerobic metabolism Muscle Smooth 04 agricultural and veterinary sciences porcine Adenosine Cell Hypoxia medicine.anatomical_structure Endocrinology chemistry iris sphincter Sphincter Female medicine.symptom Muscle Contraction Muscle contraction medicine.drug |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | Eyes are supplied O2 through the cornea and vessels of the retina and iris, which are tissues characterized by aerobic metabolism. Meanwhile, there are no reports on the association between iris sphincter contraction and aerobic metabolism. In this paper, we studied the aforementioned association. Eyes from adult pigs of either sex were obtained from a local abattoir. A muscle strip was connected to a transducer to isometrically record the tension. O2 consumption was measured using a Clark-type polarograph connected to a biological oxygen monitor. Creatine phosphate (PCr) and adenosine triphosphate (ATP) contents were measured in the muscle strips by high-performance liquid chromatography (HPLC). Iris sphincter muscles were measured in resting, contractile or hypoxic phases. Contraction was induced by hyperosmotic 65 mM KCl (H-65K+) or carbachol (CCh), and hypoxia was induced by aeration with N2 instead of O2 or by addition of sodium cyanide (NaCN). H-65K+- and CCh-induced muscle contraction, involved increasing O2 consumption. Hypoxia and NaCN significantly decreased H-65K+- and CCh-induced muscle contraction and/or O2 consumption and PCr contents. Our results suggest that the contractile behavior in porcine iris sphincter highly depends on mitogen oxidative metabolism. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|